星形胶质细胞上调基因1通过激活Wnt/β-连环蛋白信号通路促进乳腺癌细胞的增殖和侵袭。
Astrocyte elevated gene-1 promotes the proliferation and invasion of breast cancer cells by activating the Wnt/β-catenin signaling pathway.
作者信息
Li Mengxi, Dai Yuna, Wang Li, Li Lei
机构信息
Department of Breast Surgery, Affiliated Hospital of Hebei Engineering University, Handan, Hebei 056002, P.R. China.
Department of General Surgery, Affiliated Hospital of Hebei Engineering University, Handan, Hebei 056002, P.R. China.
出版信息
Oncol Lett. 2017 Apr;13(4):2385-2390. doi: 10.3892/ol.2017.5695. Epub 2017 Feb 8.
Abstract
Astrocyte elevated gene-1 (AEG1) was identified to be overexpressed in breast cancer, and to be associated with the development of breast cancer. In the present study, AEG1 was identified as highly expressed in the MCF-7, MDA-MB-231 and SK-BR-3 breast cancer cell lines and was detected in the MCF-10A normal breast epithelial cell line. The present study established an AEG1-knockdown MCF-7 cell line to investigate the expression status of certain cancer-associated proteins. Western blotting demonstrated that AEG1 may affect cancer cell proliferation and invasion via activating the Wnt/β-catenin signaling pathway, a hypothesis that has been supported by cell function tests. The results of the present study demonstrated that when AEG1 was significantly overexpressed in breast cancer cells it promoted cell proliferation and invasion via activating the Wnt/β-catenin signaling pathway. Therefore, AEG1 may serve as a novel therapeutic target in breast cancer.
摘要
星形胶质细胞上调基因1（AEG1）被证实于乳腺癌中过表达，并与乳腺癌的发展相关。在本研究中，AEG1在MCF-7、MDA-MB-231和SK-BR-3乳腺癌细胞系中高表达，而在MCF-10A正常乳腺上皮细胞系中也可检测到。本研究建立了AEG1敲低的MCF-7细胞系，以研究某些癌症相关蛋白的表达状态。蛋白质免疫印迹法表明，AEG1可能通过激活Wnt/β-连环蛋白信号通路影响癌细胞的增殖和侵袭，这一假设已得到细胞功能试验的支持。本研究结果表明，当AEG1在乳腺癌细胞中显著过表达时，它通过激活Wnt/β-连环蛋白信号通路促进细胞增殖和侵袭。因此，AEG1可能成为乳腺癌的一个新的治疗靶点。
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