Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, China.
Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, China.
J Fish Dis. 2020 May;43(5):531-540. doi: 10.1111/jfd.13146. Epub 2020 Feb 25.
Grouper iridovirus causes high mortality rates in cultured groupers, and effective treatment for grouper iridovirus infection is urgently required. Illicium verum Hook. f. is a well-known medicinal plant with a variety of biological activities. The aim of this study was to analyse the use of I. verum extracts to treat grouper iridovirus infection. The safe working concentration of each I. verum extract was identified both in vitro and in vivo as follows: I. verum aqueous extract (IVAE) ≤ 500 μg/ml; I. verum ethanol extract (IVEE) ≤ 250 μg/ml; shikimic acid (SKA) ≤ 250 μg/ml; trans-anethole (TAT) ≤ 800 μg/ml; 3,4-dihydroxybenzoic acid (DDBA) ≤ 400 μg/ml; and quercetin (QCE) ≤ 50 μg/ml. The inhibitory activity of each I. verum extract against grouper iridovirus infection was analysed using aptamer (Q2)-based fluorescent molecular probe (Q2-AFMP) and RT-qPCR. All of the I. verum extracts displayed dose-dependent antiviral activities against grouper iridovirus. Based on the achieved per cent inhibition, IVAE, IVEE, DDBA and QCE were associated with the greatest antiviral activity (all > 90%). Together, our results indicate that I. verum extracts have effective antiviral properties, making it an excellent potential source material for the development of effective treatment for grouper iridovirus infection.
石菖蒲提取物抗鱼类虹彩病毒的作用研究
石菖蒲（Ilicium verum Hook. f.）是一种传统的中药材，具有多种生物活性。本研究旨在分析石菖蒲提取物对鱼类虹彩病毒感染的治疗作用。采用细胞病变法（CPE）和实时荧光定量 PCR（RT-qPCR）检测了石菖蒲水提物（IVAE）、石菖蒲醇提物（IVEE）、莽草酸（SKA）、反式-茴香脑（TAT）、3,4-二羟基苯甲酸（DDBA）和槲皮素（QCE）对鱼类虹彩病毒感染的抑制作用。结果表明，在体外和体内，石菖蒲各提取物的安全工作浓度分别为：IVAE≤500μg/ml，IVEE≤250μg/ml，SKA≤250μg/ml，TAT≤800μg/ml，DDBA≤400μg/ml，QCE≤50μg/ml。基于适配体（Q2）荧光分子探针（Q2-AFMP）和 RT-qPCR 检测，所有石菖蒲提取物均表现出对鱼类虹彩病毒感染的剂量依赖性抑制作用。根据抑制率，IVAE、IVEE、DDBA 和 QCE 对鱼类虹彩病毒的抑制活性最强（均>90%）。综上，石菖蒲提取物具有有效的抗病毒作用，是开发鱼类虹彩病毒感染有效治疗药物的潜在良好来源。
