Gaur V P, Eldred W, Sarthy P V
Department of Ophthalmology, University of Washington, Seattle 98195.
J Neurocytol. 1988 Oct;17(5):683-92. doi: 10.1007/BF01260995.
Müller cells are the major type of glial cell in the vertebrate retina, and appear to participate in important structural and metabolic functions. Although the morphological features of Müller cells have been extensively studied, their topographic distribution across the retina has not been previously reported. We have used a Müller cell-specific monoclonal antibody, 19-33, to study the distribution of Müller cells in turtle retina. The antibody was obtained during a search for cell type-specific monoclonal antibodies in the rat retina. Immunoblotting studies show that 19-33 reacts with a 58 KDa protein that is present in Müller cells. Immunocytochemical studies with en face sections of turtle retina show that the density of Müller cells is fairly uniform across the retina although there are small regional differences. We estimate that the mean Müller cell density is about 1600 cells mm-2 of turtle retina and that each turtle retina contains about 54,000 Müller cells.
穆勒细胞是脊椎动物视网膜中主要的神经胶质细胞类型，似乎参与重要的结构和代谢功能。尽管对穆勒细胞的形态特征已进行了广泛研究，但此前尚未报道过它们在整个视网膜中的拓扑分布。我们使用一种穆勒细胞特异性单克隆抗体19 - 33来研究龟视网膜中穆勒细胞的分布。该抗体是在对大鼠视网膜中细胞类型特异性单克隆抗体的研究中获得的。免疫印迹研究表明，19 - 33与穆勒细胞中存在的一种58千道尔顿的蛋白质发生反应。对龟视网膜表面切片的免疫细胞化学研究表明，尽管存在小的区域差异，但整个视网膜中穆勒细胞的密度相当均匀。我们估计龟视网膜中穆勒细胞的平均密度约为每平方毫米1600个细胞，并且每个龟视网膜约包含54,000个穆勒细胞。
