Pino J A, Bashein G, Kenny M A
Department of Laboratory Medicine, University of Washington School of Medicine, Seattle 98195.
J Clin Monit. 1988 Jul;4(3):186-94. doi: 10.1007/BF01621815.
The Gas-STAT blood gas monitor uses fluorometric techniques to continuously monitor blood gas tensions and acid-base status in the extracorporeal perfusion circuit during cardiac surgery. We evaluated the in vitro performance of this instrument by using a tonometry loop to simulate the clinical environment and to provide controlled gas tensions and pH in the circulating fluid. In this article we report the in vitro study in which 35 Gas-STAT blood gas sensors were used to assess the precision, stability, response time, and specificity of the instrument and to confirm the sterile integrity of its flow-through cells. The blood gas monitor exhibited precision values for pH, carbon dioxide tension (PCO2), and oxygen tension (PO2) of 0.1%, 1.3%, and 1.0%, respectively; stabilities were 0.002 units/h for pH, 0.5 mm Hg/h for PCO2, and 1.4 mm Hg/h for PO2; time constants (tau, a response to within 1/e of a new gas tension, approximately 63%) were 81 seconds for PCO2 and 72 seconds for PO2. No significant interference was detected in in vitro tests of 30 drugs and metabolites typically encountered during cardiac surgery. Bacterial challenge of the flow-through cell membranes showed that they provide an effective barrier isolating the sensors from contaminants in the fluid path. Our quality control consisted of measurement of a midrange gas standard as an unknown immediately following sensor calibration; this simple program is proposed as a complement to the manufacturer's operating procedures.(ABSTRACT TRUNCATED AT 250 WORDS)
气体 STAT 血气监测仪采用荧光技术，在心脏手术期间连续监测体外循环回路中的血气张力和酸碱状态。我们通过使用一个测压回路来模拟临床环境，并在循环液中提供可控的气体张力和 pH 值，以此评估该仪器的体外性能。在本文中，我们报告了一项体外研究，其中使用 35 个气体 STAT 血气传感器来评估该仪器的精密度、稳定性、响应时间和特异性，并确认其流通池的无菌完整性。该血气监测仪的 pH、二氧化碳张力（PCO2）和氧张力（PO2）的精密度值分别为 0.1%、1.3% 和 1.0%；pH 的稳定性为 0.002 单位/小时，PCO2 为 0.5 毫米汞柱/小时，PO2 为 1.4 毫米汞柱/小时；时间常数（τ，对新气体张力达到 1/e 以内的响应，约 63%），PCO2 为 81 秒，PO2 为 72 秒。在心脏手术中常见的 30 种药物和代谢物的体外测试中未检测到明显干扰。对流通池膜进行细菌挑战试验表明，它们能提供有效的屏障，将传感器与流体路径中的污染物隔离开来。我们的质量控制包括在传感器校准后立即将一个中等范围的气体标准作为未知样品进行测量；这个简单的程序被提议作为制造商操作程序的补充。（摘要截短于 250 字）
