Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd. Nan-Tzu, Taiwan, ROC, Kaohsiung City 811.
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1508-1515. doi: 10.1099/ijsem.0.003875.
Strain TTM-71, isolated from a freshwater river in Taiwan, was characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain TTM-71 is affiliated with species in the genus . The 16S rRNA gene sequence similarity indicated that strain TTM-71 is closely related to species within the genus (94.7-95.5 % sequence similarity) and had a high sequence similarity with SR 2-06 (95.5 %). Strain TTM-71 showed 70.3 % average nucleotide identity and 24.9 % digital DNA-DNA hybridization identity with YT21. Cells were Gram-stain-negative, aerobic, motile by gliding, rod-shaped and formed beige-colored colonies. Optimal growth occurred at 20 °C, pH 8, and in the presence of 0.5 % NaCl. The major fatty acids of strain TTM-71 were iso-C, iso-C G and summed feature 3 (comprising C ω and/or C ω). The predominant hydroxy fatty acid was iso-C 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, four uncharacterized aminophospholipids, one uncharacterized aminolipid, one uncharacterized phospholipid and one uncharacterized lipid. The predominant polyamine was homospermidine. The only isoprenoid quinone was MK-7. Genomic DNA G+C content was 45.6 mol%. On the basis of the polyphasic evidence presented, strain TTM-71 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TTM-71 (=BCRC 81160=LMG 31017=KCTC 62871).
从台湾一条淡水河中分离得到的一株菌株 TTM-71,采用多相分类方法进行了特征描述。基于 16S rRNA 基因序列和最新的细菌核心基因集(92 个蛋白簇)的系统发育分析表明,菌株 TTM-71 属于 属的物种。16S rRNA 基因序列相似性表明,菌株 TTM-71 与属内的种密切相关(94.7-95.5%序列相似性),与 SR 2-06(95.5%)具有很高的序列相似性。菌株 TTM-71 与 YT21 的平均核苷酸同一性为 70.3%,数字 DNA-DNA 杂交同一性为 24.9%。细胞革兰氏染色阴性,需氧,滑行运动,杆状,形成米色菌落。菌株 TTM-71 的最佳生长条件为 20°C、pH 值 8 和 0.5%NaCl。菌株 TTM-71 的主要脂肪酸为 iso-C、iso-C G 和总和特征 3(包括 C ω和/或 C ω)。主要的羟基脂肪酸为 iso-C 3-OH。极性脂谱由磷脂酰乙醇胺、四种未鉴定的氨基磷脂、一种未鉴定的氨基脂、一种未鉴定的磷脂和一种未鉴定的脂组成。主要聚胺为腐胺。唯一的异戊烯醌为 MK-7。基因组 DNA G+C 含量为 45.6mol%。基于提供的多相证据,菌株 TTM-71 被认为代表了 属的一个新种,因此提议使用 sp. nov.作为新种的名称。模式菌株为 TTM-71(=BCRC 81160=LMG 31017=KCTC 62871)。