sp. nov., isolated from a fish pond.

Bacterial strain NST-5, isolated from a fish pond in Taiwan, was characterized using a polyphasic taxonomy approach. Results of phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain NST-5 formed a phylogenetic lineage in the genus . Analysis of 16S rRNA gene sequences showed that strain NST-5 showed the highest similarity to DK69 (94.9 %), 10Alg 130 (94.8 %) and THG-SM1 (94.8 %). Strain NST-5 showed 68.9-72.5% average nucleotide identity and 19.1-23.7% digital DNA-DNA hybridization identity with the type strains of other close related species. Cells of the strain were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and formed yellow colonies. Optimal growth occurred at 30 °C, pH 7 and with 0.5% NaCl. Strain NST-5 contained iso-C, C and iso-C as the predominant fatty acids. The major hydroxyl fatty acids were iso-C 3-OH and iso-C 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, three uncharacterized aminophospholipids, two uncharacterized phospholipids and one uncharacterized aminolipid. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 35.5 mol%. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain NST-5 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NST-5 (=BCRC 81198=LMG 31341).