Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2020 Sep;70(9):5075-5086. doi: 10.1099/ijsem.0.004384.
Bacterial strain NST-5, isolated from a fish pond in Taiwan, was characterized using a polyphasic taxonomy approach. Results of phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain NST-5 formed a phylogenetic lineage in the genus . Analysis of 16S rRNA gene sequences showed that strain NST-5 showed the highest similarity to DK69 (94.9 %), 10Alg 130 (94.8 %) and THG-SM1 (94.8 %). Strain NST-5 showed 68.9-72.5% average nucleotide identity and 19.1-23.7% digital DNA-DNA hybridization identity with the type strains of other close related species. Cells of the strain were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and formed yellow colonies. Optimal growth occurred at 30 °C, pH 7 and with 0.5% NaCl. Strain NST-5 contained iso-C, C and iso-C as the predominant fatty acids. The major hydroxyl fatty acids were iso-C 3-OH and iso-C 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, three uncharacterized aminophospholipids, two uncharacterized phospholipids and one uncharacterized aminolipid. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 35.5 mol%. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain NST-5 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NST-5 (=BCRC 81198=LMG 31341).
从台湾的一个鱼塘中分离到的细菌菌株 NST-5，采用多相分类学方法进行了特征描述。基于 16S rRNA 基因序列和 92 个蛋白聚类编码序列的系统发育分析结果表明，菌株 NST-5 形成了一个属内的系统发育分支。16S rRNA 基因序列分析表明，菌株 NST-5 与 DK69（94.9%）、10Alg 130（94.8%）和 THG-SM1（94.8%）的相似度最高。菌株 NST-5 与其他近缘 种的模式菌株的平均核苷酸同一性为 68.9-72.5%，数字 DNA-DNA 杂交同一性为 19.1-23.7%。该菌株的细胞革兰氏阴性，严格需氧，滑行运动，呈杆状，形成黄色菌落。最佳生长条件为 30°C、pH 7 和 0.5%NaCl。菌株 NST-5 含有 iso-C、C 和 iso-C 作为主要脂肪酸。主要羟基脂肪酸为 iso-C 3-OH 和 iso-C 3-OH。极性脂谱由磷脂酰乙醇胺、三种未鉴定的氨基磷脂、两种未鉴定的磷脂和一种未鉴定的氨基脂组成。主要多胺为腐胺。主要异戊烯醌为 MK-6。基因组 DNA 的 G+C 含量为 35.5 mol%。表型差异特征与系统发育推断共同表明，菌株 NST-5 应归类为一个新的属种，建议命名为 sp. nov.。其模式菌株为 NST-5（=BCRC 81198=LMG 31341）。
