Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea.
Ilseong Landscaping Co.Ltd, Jeju 63242, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2194-2203. doi: 10.1099/ijsem.0.003914. Epub 2020 Feb 20.
A Gram-reaction-negative, motile by gliding, rod-shaped bacterium, designated strain GH4-78, was isolated from the tidal mudflat of a beach in the Republic of Korea. Cells were aerobic, catalase-positive, oxidase-negative and produced cream-coloured colonies. Q-8 was the only isoprenoid quinone. The major fatty acids were summed feature 8 (C 7 and/or C 6), summed feature 3 (C ω6 and/or C ω7) and C. The major polar lipids are phosphatidylethanolamine and phosphatidylglycerol. Results of phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GH4-78 formed a distinct lineage with (98.2 % sequence similarity). The DNA G+C content was 59.9 mol%. The average nucleotide identity value with the closest relative was 82.90 %. On the basis of the results from phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain GH4-78 (=KCTC 62383=DSM 106349) represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. Moreover, the transfers of Han . 2019 to comb. nov. and Shi . 2018 to gen. nov. as comb. nov. are also proposed, with the emended description of the genus .
一株革兰氏反应阴性、可滑行运动的杆状细菌，被命名为 GH4-78 菌株，从韩国某海滩的潮汐泥滩中分离得到。细胞为需氧型，过氧化氢酶阳性，氧化酶阴性，产生奶油色菌落。Q-8 是唯一的异戊二烯醌。主要脂肪酸为 sumF 8（C7 和/或 C6）、sumF 3（Cω6 和/或 Cω7）和 C₁₆:₀。主要极性脂为磷脂酰乙醇胺和磷脂酰甘油。基于 16S rRNA 基因序列的系统发育分析结果表明，菌株 GH4-78 与 （相似度为 98.2%）形成了一个独特的分支。DNA G+C 含量为 59.9mol%。与最接近的亲缘关系的平均核苷酸同一性值为 82.90%。基于表型、化学分类、系统发育和系统基因组学分析的结果，菌株 GH4-78（=KCTC 62383=DSM 106349）代表了一个新属的新种，属于 科，提议采用新属名 ，新种名 。此外，提议将 Han. 2019 转移到 comb. nov. 和 Shi. 2018 到 gen. nov. 作为 comb. nov.，并对 进行了修正描述。
