State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, PR China.
College of Marine Life Sciences, Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2624-2631. doi: 10.1099/ijsem.0.004083. Epub 2020 Mar 5.
A Gram-stain-negative, aerobic, non-flagellated and rod- or ovoid-shaped bacterium, designated as strain S4J41, was isolated from Antarctic intertidal sediment. The isolate grew at 0-37 °C and with 0.5-10 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed Tween 80 and gelatin. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S4J41 constituted a distinct phylogenetic line within the family and was closely related with some species in the genera , , , , and with 98.6-95.7 % 16S rRNA gene sequence similarities. The major cellular fatty acids were C, summed feature 8 (C ω7 and/or C ω6) and C and the major polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. The sole respiratory quinone was Q-10. The genomic DNA G+C content of strain S4J41 was 60.3 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain S4J41 is considered to represent a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is S4J41 (=MCCC 1K03508=KCTC 62793). Moreover, the transfer of Kim . 2019 to gen. nov. as comb. nov. (type strain 318-1=JCM 30927=KCTC 72105) is also proposed.
一株革兰氏阴性、需氧、无鞭毛、杆状或卵圆形的细菌，被命名为 S4J41 菌株，从南极潮间带沉积物中分离得到。该分离株在 0-37°C 下生长，可耐受 0.5-10%（w/v）的 NaCl。它能将硝酸盐还原为亚硝酸盐，并水解吐温 80 和明胶。基于 16S rRNA 基因序列的系统发育分析表明，菌株 S4J41 构成了 科内的一个独特分支，与属内的一些种、、、、和 亲缘关系最近，与它们的 16S rRNA 基因序列相似性为 98.6-95.7%。主要的细胞脂肪酸为 C 、特征脂肪酸 8（C ω7 和/或 C ω6）和 C ，主要的极性脂为磷脂酰甘油、磷脂酰胆碱、双磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的胺脂。唯一的呼吸醌为 Q-10。菌株 S4J41 的基因组 DNA G+C 含量为 60.3mol%。根据本研究获得的系统发育、化学分类和表型数据，菌株 S4J41 代表了 科内一个新属的新种，建议将其命名为 属，新种，新种。模式菌株为 S4J41（=MCCC 1K03508=KCTC 62793）。此外，还建议将 Kim. 2019 转移到 属，作为 组合新属（模式菌株 318-1=JCM 30927=KCTC 72105）。
