gen. nov., sp. nov., isolated from a sea tidal flat and proposal of fam. nov.

A Gram-negative, moderately halophilic and facultatively aerobic bacterium, designated strain GTF13, was isolated from a sea tidal flat. Cells were curved rods and motile by a single polar flagellum showing catalase and oxidase activities. Growth was observed at 20-37 °C, pH 5.0-8.5 and 1.0-6.0 % (w/v) NaCl. Strain GTF13 contained C summed feature 3 (comprising C ω6/C ω7), summed feature 8 (comprising C ω6c/C ω7) and C 3-OH as major fatty acids and ubiquinone-9 and ubiquinone-8 as major quinones. Phosphatidylethanolamine and two unidentified phospholipids were detected as major polar lipids. The G+C content of the genomic DNA was 59.8 mol%. Strain GTF13 was most closely related to SA1, CL-33 and FR1439, belonging to different families or orders of the class , with less than 92.0 % 16S rRNA gene sequence similarities. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTF13 formed a phylogenetic lineage with the family , but the genome-based phylogenomic tree showed that strain GTF13 formed a distinct phylogenetic lineage within the order . The very low 16S rRNA gene sequence similarities and distinct phylogenetic relationships, together with distinct phenotypic and chemotaxonomic properties, served to differentiate strain GTF13 from phylogenetically closely related families. Here, strain GTF13 is proposed as a novel genus and species, for which the name gen. nov., sp. nov. is proposed, within a new family fam. nov. of the order . The type strain is GTF13 (=KACC 19788=JCM 32043).