Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Department of Civil and Environmental Engineering, Chung-Ang University, Seoul 06974, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2239-2246. doi: 10.1099/ijsem.0.003976. Epub 2020 Feb 11.
A Gram-negative, moderately halophilic and facultatively aerobic bacterium, designated strain GTF13, was isolated from a sea tidal flat. Cells were curved rods and motile by a single polar flagellum showing catalase and oxidase activities. Growth was observed at 20-37 °C, pH 5.0-8.5 and 1.0-6.0 % (w/v) NaCl. Strain GTF13 contained C summed feature 3 (comprising C ω6/C ω7), summed feature 8 (comprising C ω6c/C ω7) and C 3-OH as major fatty acids and ubiquinone-9 and ubiquinone-8 as major quinones. Phosphatidylethanolamine and two unidentified phospholipids were detected as major polar lipids. The G+C content of the genomic DNA was 59.8 mol%. Strain GTF13 was most closely related to SA1, CL-33 and FR1439, belonging to different families or orders of the class , with less than 92.0 % 16S rRNA gene sequence similarities. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTF13 formed a phylogenetic lineage with the family , but the genome-based phylogenomic tree showed that strain GTF13 formed a distinct phylogenetic lineage within the order . The very low 16S rRNA gene sequence similarities and distinct phylogenetic relationships, together with distinct phenotypic and chemotaxonomic properties, served to differentiate strain GTF13 from phylogenetically closely related families. Here, strain GTF13 is proposed as a novel genus and species, for which the name gen. nov., sp. nov. is proposed, within a new family fam. nov. of the order . The type strain is GTF13 (=KACC 19788=JCM 32043).
一株革兰氏阴性、中度嗜盐、兼性需氧细菌，被命名为 GTF13 菌株，从一个潮滩中分离得到。细胞为弯曲的杆状，通过单个极生鞭毛运动，具有过氧化氢酶和氧化酶活性。在 20-37℃、pH5.0-8.5 和 1.0-6.0%（w/v）NaCl 条件下生长。GTF13 菌株含有 C 总和特征 3（包含 C ω6/C ω7）、C 总和特征 8（包含 C ω6c/C ω7）和 C 3-OH 作为主要脂肪酸，以及泛醌-9 和泛醌-8 作为主要醌类。检测到磷脂酰乙醇胺和两种未鉴定的磷脂作为主要极性脂。基因组 DNA 的 G+C 含量为 59.8 mol%。GTF13 菌株与属于不同科或目的 SA1、CL-33 和 FR1439 最为密切相关，16S rRNA 基因序列相似性低于 92.0%。基于 16S rRNA 基因序列的系统发育分析表明，GTF13 菌株与科内的一个家族形成一个系统发育分支，但基于基因组的系统发育树表明，GTF13 菌株在目内形成一个独特的系统发育分支。非常低的 16S rRNA 基因序列相似性和独特的系统发育关系，以及明显的表型和化学分类特性，使 GTF13 菌株与系统发育上密切相关的家族区分开来。在这里，GTF13 菌株被提议为一个新属和新种，建议使用新的属名 gen. nov. 和新种名 sp. nov.，隶属于目内的一个新科 fam. nov.。模式菌株为 GTF13（=KACC 19788=JCM 32043）。
