Applied Biotechnology Research Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, United Kingdom. School of Life Sciences, Medical School, University of Nottingham, Nottigham, United Kingdom.
Biomed Mater. 2020 Jul 1;15(4):045024. doi: 10.1088/1748-605X/ab797b.
The biocompatibility and neuron regenerating properties of various bioactive glass (BG)/polyhydroxyalkanoate (PHA) blend composites were assessed in order to study their suitability for peripheral nerve tissue applications, specifically as lumen structures for nerve guidance conduits. BG/PHA blend composites were fabricated using Bioactive glass® 45 S5 (BG1) and BG 1393 (BG2) with the 25:75 poly(3-hydroxyoctanoate/poly3-hydroxybutyrate), 25:75 P(3HO)/P(3HB) blend (PHA blend). Various concentrations of each BG (0.5 wt%, 1.0 wt% and 2.5 wt%) were used to determine the effect of BG on neuronal growth and differentiation, in single culture using NG108-15 neuronal cells and in a co-culture along with RN22 Schwann cells. NG108-15 cells exhibited good growth and differentiation on all the PHA blend composites showing that both BGs have good biocompatibility at 0.5 wt%, 1.0 wt% and 2.5 wt% within the PHA blend. The Young's modulus values displayed by all the PHA blend/BG composites ranged from 385.6 MPa to 1792.6 MPa, which are able to provide the required support and protective effect for the regeneration of peripheral nerves. More specifically, the tensile strength obtained in the PHA blend/BG1 (1.0 wt%) (10.0 ± 0.6 MPa) was found to be similar to that of the rabbit peroneal nerve. This composite also exhibited the best biological performance in supporting growth and neuronal differentiation among all the substrates. The neurite extension on this composite was found to be remarkable with the neurites forming a complex connection network.
为了研究其在周围神经组织中的适用性，特别是作为神经引导导管的管腔结构，评估了各种生物活性玻璃（BG）/聚羟基烷酸酯（PHA）共混复合材料的生物相容性和神经元再生性能。使用 Bioactive glass® 45 S5（BG1）和 BG 1393（BG2）与 25:75 聚（3-羟基辛烷酸酯/聚 3-羟基丁酸酯）、25:75 P（3HO）/P（3HB）共混物（PHA 共混物）制备 BG/PHA 共混复合材料。使用各种浓度的每种 BG（0.5wt%、1.0wt%和 2.5wt%）来确定 BG 对神经元生长和分化的影响，在 NG108-15 神经元细胞的单一培养中和与 RN22 施万细胞的共培养中进行。NG108-15 细胞在所有 PHA 共混复合材料上均表现出良好的生长和分化，表明在 PHA 共混物中，两种 BG 在 0.5wt%、1.0wt%和 2.5wt%时均具有良好的生物相容性。所有 PHA 共混物/BG 复合材料的杨氏模量值在 385.6MPa 到 1792.6MPa 之间，能够为周围神经的再生提供所需的支持和保护作用。更具体地说，在 PHA 共混物/BG1（1.0wt%）（10.0±0.6MPa）中获得的拉伸强度与兔腓神经相似。该复合材料在支持生长和神经元分化方面表现出最佳的生物学性能，在所有基质中。在这种复合材料上的神经突延伸被发现是显著的，神经突形成复杂的连接网络。
