MolecuLight Inc., Toronto, ON M5G 1T6, Canada.
Department of Honors Studies, Texas Tech University, Lubbock, TX 79409, USA.
Future Microbiol. 2020 Mar;15:319-332. doi: 10.2217/fmb-2019-0279. Epub 2020 Feb 26.
Fluorescence imaging can visualize polymicrobial populations in chronic and acute wounds based on porphyrin fluorescence. We investigated the fluorescent properties of specific wound pathogens and the fluorescence detected from bacteria in biofilm. Utilizing Remel Porphyrin Test Agar, 32 bacterial and four yeast species were examined for red fluorescence under 405 nm violet light illumination. Polymicrobial biofilms, supplemented with δ-aminolevulinic acid, were investigated similarly. A total of 28/32 bacteria, 1/4 yeast species and polymicrobial biofilms produced red fluorescence, in agreement with their known porphyrin production abilities. These results identify common wound pathogens capable of producing porphyrin-specific fluorescence and support clinical observations using fluorescence imaging to detect pathogenic bacteria in chronic wounds.
荧光成像是基于卟啉荧光来可视化慢性和急性伤口中的多微生物群体。我们研究了特定伤口病原体的荧光特性和生物膜中细菌的荧光检测。利用雷梅尔卟啉测试琼脂，在 405nm 紫光照射下，检查了 32 种细菌和 4 种酵母属的红色荧光。同样，也研究了补充 δ-氨基酮戊酸的多微生物生物膜。总共 28/32 种细菌、1/4 种酵母属和多微生物生物膜产生红色荧光，这与它们已知的卟啉产生能力一致。这些结果确定了能够产生卟啉特异性荧光的常见伤口病原体，并支持使用荧光成像来检测慢性伤口中致病性细菌的临床观察。
