Mechanisms exploration of herbal pair of HuangQi-DanShen on cerebral ischemia based on metabonomics and network pharmacology.

ETHNOPHARMACOLOGICAL RELEVANCE

The herbal pair of HuangQi-DanShen (HD) is frequently used for treating brain injury caused by cerebral ischemia (CI) in traditional Chinese medicine (TCM).

AIM OF THE STUDY

The present work was designed to reveal the active mechanism of HD against CI.

MATERIALS AND METHODS

In our work, an integrated approach combined H-NMR based metabonomics and network pharmacology was applied to decipher the protection of HD against MCAO (middle cerebral artery occlusion)-induced CI rats. Meanwhile, the indicator of neurological deficit and TTC staining were used to estimate the efficacy of HD.

RESULTS

The results of neurological deficit test and TTC staining suggested HD could improve the brain injury in CI rats. The metabonomic result indicated that HD could significantly ameliorate 8 serum metabolites in CI rats, which were linked 71 corresponding targeted proteins obtained by Metscape. In addition, 84 targets related HD against CI were obtained by network pharmacology. At last, 5 important targets were screened as hopeful targets for the treatment of CI through integrating them.

CONCLUSION

The integrated method coupled H-NMR based metabonomics with network pharmacology provided the insights into the mechanisms of TCM in treating CI.