Ahmad Mohammad Margoob, Parveen Farah, Akhter Naseem, Siddiqui Jamshaid Ahmad, Shukla Nootan Kumar, Husain Syed Akhtar
Department of Biotechnology, Jamia Millia Islamia, New Delhi, India.
Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
Asian Pac J Cancer Prev. 2020 Feb 1;21(2):301-308. doi: 10.31557/APJCP.2020.21.2.301.
Cytokines are the key regulator molecules that modulate immune response. Tumor necrosis factor (TNF- α-308 G/A and TNF-β +252 A/G ) are inflammatory cytokine that control the progression of several types of cancer. They play a vital role in both tumor progression and destruction based on their concentrations. The role of TNF-α-308 G/A and TNF-β +252 A/G gene polymorphism in the etiology of breast cancer (BC) is not clearly understood. Therefore, present study investigates the association of TNF-α -308 G/A and TNF-β +252 A/G and the clinical features with Breast cancer patients.
In a case- control study, we have investigated 150 breast cancer patients and 300 age and ethnically matched healthy controls for duration of 3 years from North India. Promoter polymorphisms of tumor necrosis factor gene (TNF-α -308 G/A and TNF-β +252 A/G) were genotyped using allele specific oligonucleotide polymerase chain reaction ASO and restriction fragment length polymorphism (PCR-RFLP). The associations were evaluated by calculating the pooled odds ratio (OR) with 95% confidence interval (95% CI) using SPSS.
Patients with different clinico-pathological variables and healthy controls were analyzed. Significant association was observed in A allele of TNF-α -308 G/A in breast cancer patients as compared to healthy controls (p<0.0001). However, no association was seen in TNF-β +252 A/G both at genotypic and allelic level. The GG genotype of TNF-β +252A/G is higher in grades III (p<0.01) patients.
Our results suggest that TNF-α-308G/A polymorphism showed significant association with breast cancer patients.
细胞因子是调节免疫反应的关键调节分子。肿瘤坏死因子（TNF-α-308 G/A和TNF-β +252 A/G）是控制几种癌症进展的炎性细胞因子。它们根据浓度在肿瘤进展和破坏中都起着至关重要的作用。TNF-α-308 G/A和TNF-β +252 A/G基因多态性在乳腺癌（BC）病因中的作用尚不清楚。因此，本研究调查了TNF-α -308 G/A和TNF-β +252 A/G与乳腺癌患者的临床特征之间的关联。
在一项病例对照研究中，我们对来自印度北部的150名乳腺癌患者和300名年龄和种族匹配的健康对照进行了为期3年的调查。使用等位基因特异性寡核苷酸聚合酶链反应ASO和限制性片段长度多态性（PCR-RFLP）对肿瘤坏死因子基因（TNF-α -308 G/A和TNF-β +252 A/G）的启动子多态性进行基因分型。使用SPSS通过计算合并比值比（OR）和95%置信区间（95%CI）来评估关联。
对具有不同临床病理变量的患者和健康对照进行了分析。与健康对照相比，乳腺癌患者中TNF-α -308 G/A的A等位基因存在显著关联（p<0.0001）。然而，在TNF-β +252 A/G的基因型和等位基因水平均未发现关联。TNF-β +252A/G的GG基因型在III级（p<0.01）患者中更高。
我们的结果表明，TNF-α-308G/A多态性与乳腺癌患者存在显著关联。
