Cullin 4B的上调促进胃癌并预示不良预后。
Upregulation of Cullin 4B Promotes Gastric Cancer and Predicts Poor Prognosis.
作者信息
Wu Ping, Hu Haolin, Li Jinwen, Gong Wei
机构信息
Department of Oncology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, 441021, People's Republic of China.
Department of Surgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, 441021, People's Republic of China.
出版信息
Onco Targets Ther. 2020 Feb 11;13:1235-1243. doi: 10.2147/OTT.S234706. eCollection 2020.
AIM
Cullin 4B (CUL4B) is a member of the cullin ubiquitin-ligase family, which participates in proteolysis. Aberrant CUL4B expression has been shown in many malignancies. This study aimed to elucidate oncogenic role of CUL4B in gastric cancer (GC).
METHODS
CUL4B expression in GC tissues was examined by RT-PCR and immunohistochemistry. The proliferation, invasion and tumorigenicity of GC cells with CUL4B overexpression or knockdown were evaluated.
RESULTS
CUL4B expression significantly increased in GC tissues, and was correlated to UICC stage and differentiation of GC, as well as poor overall survival and disease-free survival. Both univariate and multivariate analysis identified CUL4B as an independent predictor for GC patient prognosis. In addition, CUL4B promoted GC cell proliferation and invasion in vitro and tumor formation in vivo.
CONCLUSION
CUL4B is overexpressed to promote GC development and progression. CUL4B is a promising prognostic marker and therapeutic target for GC.
目的
Cullin 4B（CUL4B）是泛素连接酶家族的成员，参与蛋白质水解过程。在许多恶性肿瘤中均已发现CUL4B表达异常。本研究旨在阐明CUL4B在胃癌（GC）中的致癌作用。
方法
采用逆转录-聚合酶链反应（RT-PCR）和免疫组织化学方法检测GC组织中CUL4B的表达。评估CUL4B过表达或敲低的GC细胞的增殖、侵袭和致瘤性。
结果
GC组织中CUL4B表达显著增加，且与国际抗癌联盟（UICC）分期、GC分化程度相关，也与总生存期和无病生存期较差有关。单因素和多因素分析均确定CUL4B为GC患者预后的独立预测因子。此外，CUL4B在体外促进GC细胞增殖和侵袭，在体内促进肿瘤形成。
结论
CUL4B过表达促进GC的发生和发展。CUL4B是一种有前景的GC预后标志物和治疗靶点。
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