Song Kun, Hao Jingduo, Ge Zuyin, Chen Pushi
Department of General Surgery, People's Hospital of Zhenhai, Ningbo, Zhejiang 315202, China.
Health Management Center, Ningbo First Hospital, Ningbo, Zhejiang 315000, China.
J Oncol. 2020 Feb 13;2020:3761535. doi: 10.1155/2020/3761535. eCollection 2020.
Transcription factor sex-determining region Y-box 2 (SOX2) involves in the maintenance of cancer stem cells. However, the role of SOX2 in colorectal cancer (CRC) remains unclear. This study was conducted to investigate the effect of SOX2 on CRC. Studies were searched using electronic databases. The combined odds ratios (ORs) or hazard ratios (HRs: multivariate Cox survival analysis) with their 95% confidence intervals (CIs) were calculated. The Cancer Genome Atlas (TCGA) and GEO datasets were further applied to validate the survival effect. The functional analysis of SOX2 was investigated. In this work, 13 studies including 2337 patients were identified, and validation data were enrolled from TCGA and GEO datasets. SOX2 expression was not significantly related to age, gender, microsatellite instability (MSI) status, clinical stage, histological grade, tumor size, pT-stage, lymph node metastasis, distal metastasis, and cancer-specific survival (CSS) but was correlated with worse overall survival (OS: = 536 patients) ( < 0.05). Furthermore, TCGA data demonstrated similar results, with no significant correlation between SOX2 and pathological characteristics. Further validation data (OS: = 1408 and disease-free survival (DFS): = 1367) showed that SOX2 expression was correlated with worse OS (HR = 1.35, 95% CI: 1.11-1.65, =0.004) and DFS (HR = 1.30, 95% CI: 1.04-1.62, =0.02). The functional analyses showed that SOX2 involved in cell-cell junction, focal adhesion, extracellular matrix- (ECM-) receptor interaction, and MAP kinase activity. Our findings suggest that SOX2 expression may be correlated with the worse prognosis of CRC.
转录因子性别决定区Y盒2（SOX2）参与癌症干细胞的维持。然而，SOX2在结直肠癌（CRC）中的作用仍不清楚。本研究旨在探讨SOX2对结直肠癌的影响。通过电子数据库检索相关研究。计算合并比值比（OR）或风险比（HR：多变量Cox生存分析）及其95%置信区间（CI）。进一步应用癌症基因组图谱（TCGA）和GEO数据集验证生存效应。对SOX2进行功能分析。在本研究中，共纳入13项研究，涉及2337例患者，并从TCGA和GEO数据集中获取验证数据。SOX2表达与年龄、性别、微卫星不稳定性（MSI）状态、临床分期、组织学分级、肿瘤大小、pT分期、淋巴结转移、远处转移及癌症特异性生存（CSS）均无显著相关性，但与较差的总生存（OS：n = 536例患者）相关（P < 0.05）。此外，TCGA数据显示了类似结果，SOX2与病理特征之间无显著相关性。进一步的验证数据（OS：n = 1408例和无病生存（DFS）：n = 1367例）表明，SOX2表达与较差的OS（HR = 1.35，95%CI：1.11 - 1.65，P = 0.004）和DFS（HR = 1.30，95%CI：1.04 - 1.62，P = 0.02）相关。功能分析表明，SOX2参与细胞间连接、粘着斑、细胞外基质（ECM）-受体相互作用及丝裂原活化蛋白激酶活性。我们的研究结果表明，SOX2表达可能与结直肠癌的预后较差相关。
