Zeng Wen, Lei Qiaoling, Ma Jiao, Gao Shuqiang, Ju Rong
Department of Neonatology, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Front Cell Neurosci. 2021 Feb 18;15:638351. doi: 10.3389/fncel.2021.638351. eCollection 2021.
Brain microvascular endothelial cells (BMECs) are a major component of the blood-brain barrier that maintains brain homeostasis. Preserving and restoring the normal biological functions of BMECs can reverse or reduce brain injury. Endothelial progenitor cells (EPCs) may promote brain vascular remodeling and restore normal endothelial function. As a novel vehicle for cell-cell communication, microvesicles (MVs) have varied biological functions. The present study investigated the biological effects of EPC-derived MVs (EPC-MVs) on BMECs . We isolated MVs from the supernatant of EPCs in a serum-depleted medium. BMECs were cultured alone or in the presence of EPC-MVs. BMEC viability and proliferation were evaluated with the Cell Counting Kit-8 and by flow cytometry, and the proangiogenic effect of EPC-MVs on BMECs was assessed with the transwell migration, wound healing, and tube formation assays. Our results showed that EPC-derived MVs labeled with DiI were internalized by cultured BMECs; this enhanced BMEC viability and promoted their proliferation. EPC-MVs also stimulated migration and tube formation in BMECs. These results demonstrate that EPC-derived MVs exert a proangiogenic effect on BMECs, which has potential applications in cell-free therapy for brain injury.
脑微血管内皮细胞（BMECs）是维持脑内环境稳定的血脑屏障的主要组成部分。维持和恢复BMECs的正常生物学功能可以逆转或减轻脑损伤。内皮祖细胞（EPCs）可能促进脑血管重塑并恢复正常的内皮功能。作为一种新型的细胞间通讯载体，微泡（MVs）具有多种生物学功能。本研究调查了EPC衍生的微泡（EPC-MVs）对BMECs的生物学作用。我们在无血清培养基中从EPCs的上清液中分离出微泡。BMECs单独培养或在EPC-MVs存在的情况下培养。使用细胞计数试剂盒-8和流式细胞术评估BMECs的活力和增殖，并通过transwell迁移、伤口愈合和管形成试验评估EPC-MVs对BMECs的促血管生成作用。我们的结果表明，用DiI标记的EPC衍生的微泡被培养的BMECs内化；这增强了BMECs的活力并促进了它们的增殖。EPC-MVs还刺激了BMECs的迁移和管形成。这些结果表明，EPC衍生的微泡对BMECs具有促血管生成作用，这在脑损伤的无细胞治疗中具有潜在应用。
