• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

线粒体功能障碍可能是苯扎氯铵产生有害影响的主要原因之一。

Mitochondrial Dysfunctions May Be One of the Major Causative Factors Underlying Detrimental Effects of Benzalkonium Chloride.

机构信息

Federal Research Center "Fundamentals of Biotechnology", Russian Academy of Sciences, Leninsky pr. 33, 119071 Moscow, Russia.

Caspian Institute of Biological Resources of the Russian Academy of Sciences, M. Gadzhiev st. 45, Makhachkala, 367025, Russia.

出版信息

Oxid Med Cell Longev. 2020 Feb 10;2020:8956504. doi: 10.1155/2020/8956504. eCollection 2020.

DOI:10.1155/2020/8956504
PMID:32104543
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7035552/
Abstract

Benzalkonium chloride (BAC) is currently the most commonly used antimicrobial preservative in ophthalmic solutions, nasal sprays, and cosmetics. However, a large number of clinical and experimental investigations showed that the topical administration of BAC-containing eye drops could cause a variety of ocular surface changes, from ocular discomfort to potential risk for future glaucoma surgery. BAC-containing albuterol may increase the risk of albuterol-related systemic adverse effects. BAC, commonly present in personal care products, in cosmetic products can induce irritation and dose-dependent changes in the cell morphology. The cationic nature of BAC (it is a quaternary ammonium) suggests that one of the major targets of BAC in the cell may be mitochondria, the only intracellular compartment charged negatively. However, the influence of BAC on mitochondria has not been clearly understood. Here, the effects of BAC on energy parameters of rat liver mitochondria as well as on yeast cells were examined. BAC, being a "weaker" uncoupler, potently inhibited respiration in state 3, diminished the mitochondrial membrane potential, caused opening of the Ca2+/Pi-dependent pore, blocked ATP synthesis, and promoted HO production by mitochondria. BAC triggered oxidative stress and mitochondrial fragmentation in yeast cells. BAC-induced oxidative stress in mitochondria and yeast cells was almost totally prevented by the mitochondria-targeted antioxidant SkQ1; the protective effect of SkQ1 on mitochondrial fragmentation was only partial. Collectively, these data showed that BAC acts adversely on cell bioenergetics (especially on ATP synthesis) and mitochondrial dynamics and that its prooxidant effect can be partially prevented by the mitochondria-targeted antioxidant SkQ1.

摘要

苯扎氯铵（BAC）目前是眼科溶液、鼻腔喷雾剂和化妆品中最常用的抗菌防腐剂。然而，大量的临床和实验研究表明，含 BAC 的眼药水局部给药可引起各种眼表面变化，从眼部不适到未来青光眼手术的潜在风险。含 BAC 的沙丁胺醇可能会增加与沙丁胺醇相关的全身不良反应的风险。常见于个人护理产品中的 BAC，在化妆品中可引起刺激和剂量依赖性的细胞形态变化。BAC 的阳离子性质（它是一种季铵盐）表明 BAC 在细胞中的主要靶标之一可能是线粒体，线粒体是唯一带负电荷的细胞内隔室。然而，BAC 对线粒体的影响尚未被清楚理解。在这里，研究了 BAC 对大鼠肝线粒体能量参数以及酵母细胞的影响。BAC 作为一种“较弱”的解偶联剂，强烈抑制了状态 3 的呼吸，降低了线粒体膜电位，导致 Ca2+/Pi 依赖性孔开放，阻断了 ATP 的合成，并促进了线粒体的 HO 生成。BAC 引发了酵母细胞中的氧化应激和线粒体碎片化。BAC 诱导的线粒体和酵母细胞中的氧化应激几乎完全被线粒体靶向抗氧化剂 SkQ1 所阻止；SkQ1 对线粒体碎片化的保护作用仅部分有效。总之，这些数据表明 BAC 对细胞生物能学（特别是对 ATP 合成）和线粒体动力学产生不利影响，其促氧化作用可部分被线粒体靶向抗氧化剂 SkQ1 所阻止。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/145c0149291e/OMCL2020-8956504.011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/dbd07a17718e/OMCL2020-8956504.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/dce8c59e3eaa/OMCL2020-8956504.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/6603b6dfef4c/OMCL2020-8956504.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/3fa251d7eaea/OMCL2020-8956504.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/04f478952985/OMCL2020-8956504.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/800b88a1d494/OMCL2020-8956504.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/927b1125d849/OMCL2020-8956504.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/dc8758b94c70/OMCL2020-8956504.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/08a0f3289a26/OMCL2020-8956504.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/4b152fece02a/OMCL2020-8956504.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/145c0149291e/OMCL2020-8956504.011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/dbd07a17718e/OMCL2020-8956504.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/dce8c59e3eaa/OMCL2020-8956504.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/6603b6dfef4c/OMCL2020-8956504.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/3fa251d7eaea/OMCL2020-8956504.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/04f478952985/OMCL2020-8956504.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/800b88a1d494/OMCL2020-8956504.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/927b1125d849/OMCL2020-8956504.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/dc8758b94c70/OMCL2020-8956504.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/08a0f3289a26/OMCL2020-8956504.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/4b152fece02a/OMCL2020-8956504.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0b/7035552/145c0149291e/OMCL2020-8956504.011.jpg

相似文献

1
Mitochondrial Dysfunctions May Be One of the Major Causative Factors Underlying Detrimental Effects of Benzalkonium Chloride.线粒体功能障碍可能是苯扎氯铵产生有害影响的主要原因之一。
Oxid Med Cell Longev. 2020 Feb 10;2020:8956504. doi: 10.1155/2020/8956504. eCollection 2020.
2
New Data on Effects of SkQ1 and SkQT1 on Rat Liver Mitochondria and Yeast Cells.SkQ1和SkQT1对大鼠肝脏线粒体及酵母细胞作用的新数据
Biochemistry (Mosc). 2018 May;83(5):552-561. doi: 10.1134/S0006297918050085.
3
SkQ3 Exhibits the Most Pronounced Antioxidant Effect on Isolated Rat Liver Mitochondria and Yeast Cells.SkQ3 在离体大鼠肝线粒体和酵母细胞中表现出最显著的抗氧化作用。
Int J Mol Sci. 2024 Jan 16;25(2):1107. doi: 10.3390/ijms25021107.
4
Mitochondria-targeted antioxidant SKQ1 protects cornea from oxidative damage induced by ultraviolet irradiation and mechanical injury.线粒体靶向抗氧化剂SKQ1可保护角膜免受紫外线照射和机械损伤诱导的氧化损伤。
BMC Ophthalmol. 2018 Dec 27;18(1):336. doi: 10.1186/s12886-018-0996-7.
5
Antioxidant mechanism of mitochondria-targeted plastoquinone SkQ1 is suppressed in aglycemic HepG2 cells dependent on oxidative phosphorylation.靶向于线粒体的质体醌 SkQ1 的抗氧化机制在依赖于氧化磷酸化的低糖 HepG2 细胞中被抑制。
Biochim Biophys Acta Bioenerg. 2017 Sep;1858(9):750-762. doi: 10.1016/j.bbabio.2017.05.005. Epub 2017 May 26.
6
An attempt to prevent senescence: a mitochondrial approach.一种预防衰老的尝试：线粒体途径。
Biochim Biophys Acta. 2009 May;1787(5):437-61. doi: 10.1016/j.bbabio.2008.12.008. Epub 2008 Dec 29.
7
Impact of dorzolamide, benzalkonium-preserved dorzolamide and benzalkonium-preserved brinzolamide on selected biomarkers of oxidative stress in the tear film.多佐胺、苯扎氯铵保存的多佐胺和苯扎氯铵保存的布林佐胺对泪膜中氧化应激相关生物标志物的影响。
BMC Ophthalmol. 2021 Sep 1;21(1):319. doi: 10.1186/s12886-021-02079-y.
8
Mitochondria-targeted plastoquinone derivatives as tools to interrupt execution of the aging program. 4. Age-related eye disease. SkQ1 returns vision to blind animals.线粒体靶向质体醌衍生物作为中断衰老程序执行的工具。4. 年龄相关性眼病。SkQ1使失明动物恢复视力。
Biochemistry (Mosc). 2008 Dec;73(12):1317-28. doi: 10.1134/s0006297908120043.
9
Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film.前列腺素 F2α 类似物对泪膜中选定氧化应激参数的影响。
Medicina (Kaunas). 2019 Jul 11;55(7):366. doi: 10.3390/medicina55070366.
10
Mitochondria-targeted plastoquinone derivatives as tools to interrupt execution of the aging program. 3. Inhibitory effect of SkQ1 on tumor development from p53-deficient cells.线粒体靶向的质体醌衍生物作为中断衰老程序执行的工具。3. SkQ1对p53缺陷细胞肿瘤发生的抑制作用。
Biochemistry (Mosc). 2008 Dec;73(12):1300-16. doi: 10.1134/s0006297908120031.

引用本文的文献

1
Topical Ocular Drug Delivery: The Impact of Permeation Enhancers.眼部局部给药：渗透促进剂的影响
Pharmaceutics. 2025 Mar 31;17(4):447. doi: 10.3390/pharmaceutics17040447.
2
Is it time to re-evaluate exposure risks to quaternary ammonium compounds as disinfectants?是时候重新评估季铵化合物作为消毒剂的暴露风险了吗？
Curr Res Microb Sci. 2024 Jul 2;7:100258. doi: 10.1016/j.crmicr.2024.100258. eCollection 2024.
3
Genome-wide RNA sequencing of ocular fibroblasts from glaucomatous and normal eyes: Implications for glaucoma management.

本文引用的文献

1
SkQThy, a novel and promising mitochondria-targeted antioxidant.SkQThy，一种新型且有前景的线粒体靶向抗氧化剂。
Mitochondrion. 2019 Nov;49:206-216. doi: 10.1016/j.mito.2019.09.001. Epub 2019 Sep 6.
2
The use of preservatives in dry eye drops.干眼症滴眼液中防腐剂的使用。
Clin Ophthalmol. 2019 Aug 1;13:1409-1425. doi: 10.2147/OPTH.S211611. eCollection 2019.
3
Tear Film Osmolarity, Ocular Surface Disease and Glaucoma: A Review.泪膜渗透压、眼表面疾病和青光眼：综述。
对青光眼和正常眼中的眼纤维细胞进行全基因组 RNA 测序：对青光眼管理的启示。
PLoS One. 2024 Jul 11;19(7):e0307227. doi: 10.1371/journal.pone.0307227. eCollection 2024.
4
The new preservative-free ophthalmic formulation of bilastine 0.6% preserves the ocular surface epithelial integrity in a comparative in vitro study.一项比较性的体外研究表明，新型无防腐剂苯海拉明滴眼液（0.6%）可维持眼表面上皮细胞的完整性。
Sci Rep. 2024 Apr 26;14(1):9598. doi: 10.1038/s41598-024-59190-5.
5
The Optic Nerve at Stake: Update on Environmental Factors Modulating Expression of Leber's Hereditary Optic Neuropathy.视神经面临风险：调节Leber遗传性视神经病变表达的环境因素最新进展
Biomedicines. 2024 Mar 6;12(3):584. doi: 10.3390/biomedicines12030584.
6
Docosahexaenoic acid alleviates LPS-induced cytotoxicity in HL-1 cardiac cells via improving stress-induced mitochondrial fragmentation.二十二碳六烯酸通过改善应激诱导的线粒体分裂减轻脂多糖诱导的HL-1心肌细胞毒性。
Heliyon. 2023 Nov 20;9(12):e22465. doi: 10.1016/j.heliyon.2023.e22465. eCollection 2023 Dec.
7
Quaternary Ammonium Compounds: A Chemical Class of Emerging Concern.季铵化合物：一类新兴关注的化学物质。
Environ Sci Technol. 2023 May 23;57(20):7645-7665. doi: 10.1021/acs.est.2c08244. Epub 2023 May 8.
8
Cytotoxicity, Mitochondrial Functionality, and Redox Status of Human Conjunctival Cells after Short and Chronic Exposure to Preservative-Free Bimatoprost 0.03% and 0.01%: An In Vitro Comparative Study.人眼结合膜细胞在短期和慢性接触无防腐剂 0.03%和 0.01%贝美前列素后的细胞毒性、线粒体功能和氧化还原状态：一项体外比较研究。
Int J Mol Sci. 2022 Nov 15;23(22):14113. doi: 10.3390/ijms232214113.
9
Quaternary ammonium compounds in hypersensitivity reactions.超敏反应中的季铵化合物。
Front Toxicol. 2022 Sep 16;4:973680. doi: 10.3389/ftox.2022.973680. eCollection 2022.
10
Benzalkonium Chloride, Even at Low Concentrations, Deteriorates Intracellular Metabolic Capacity in Human Conjunctival Fibroblasts.即使在低浓度下，苯扎氯铵也会降低人结膜成纤维细胞的细胞内代谢能力。
Biomedicines. 2022 Sep 18;10(9):2315. doi: 10.3390/biomedicines10092315.
Curr Med Chem. 2019;26(22):4241-4252. doi: 10.2174/0929867326666190725160621.
4
Modeling human disease in yeast: recreating the PI3K-PTEN-Akt signaling pathway in Saccharomyces cerevisiae.在酵母中模拟人类疾病：在酿酒酵母中重建 PI3K-PTEN-Akt 信号通路。
Int Microbiol. 2020 Jan;23(1):75-87. doi: 10.1007/s10123-019-00082-4. Epub 2019 Jun 19.
5
Multiomics Investigation Reveals Benzalkonium Chloride Disinfectants Alter Sterol and Lipid Homeostasis in the Mouse Neonatal Brain.多组学研究揭示苯扎氯铵消毒剂改变小鼠新生大脑中的甾醇和脂质稳态。
Toxicol Sci. 2019 Sep 1;171(1):32-45. doi: 10.1093/toxsci/kfz139.
6
Tear Film, Conjunctival and Corneal Modifications Induced by Glaucoma Treatment.青光眼治疗引起的泪膜、结膜和角膜改变。
Curr Med Chem. 2019;26(22):4253-4261. doi: 10.2174/0929867326666190517111823.
7
Effects of benzalkonium chloride on cell viability, inflammatory response, and oxidative stress of human alveolar epithelial cells cultured in a dynamic culture condition.苯扎氯铵对动态培养条件下人肺泡上皮细胞活力、炎症反应和氧化应激的影响。
Toxicol In Vitro. 2019 Sep;59:221-227. doi: 10.1016/j.tiv.2019.04.027. Epub 2019 Apr 25.
8
Benzalkonium Chlorides: Uses, Regulatory Status, and Microbial Resistance.苯扎氯铵：用途、监管状况和微生物耐药性。
Appl Environ Microbiol. 2019 Jun 17;85(13). doi: 10.1128/AEM.00377-19. Print 2019 Jul 1.
9
Effect of benzalkonium chloride on dentin bond strength and endogenous enzymatic activity.苯扎氯铵对牙本质粘结强度和内源性酶活性的影响。
J Dent. 2019 Jun;85:25-32. doi: 10.1016/j.jdent.2019.04.008. Epub 2019 Apr 15.
10
Benzalkonium Chloride Demonstrates Concentration-Dependent Antiviral Activity Against Adenovirus .苯扎氯铵对腺病毒表现出浓度依赖性的抗病毒活性。
J Ocul Pharmacol Ther. 2019 Jun;35(5):311-314. doi: 10.1089/jop.2018.0145. Epub 2019 Apr 10.