Proctology Department, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China.
Graduate College, Youjiang Medical University for Nationalities, Baise, Guangxi, China.
Biomed Res Int. 2020 Feb 10;2020:4914707. doi: 10.1155/2020/4914707. eCollection 2020.
. The biological roles of microRNA-654-5p (miR-654-5p) in cancers have been previously reported. However, its role in colorectal cancer (CRC) remains largely unknown. The purpose of this work was to investigate the roles and associated mechanisms in CRC.
Quantitative Real-Time PCR (qRT-PCR) was utilized to explore the expression pattern of miR-654-5p in CRC cells. Cell Counting Kit-8 (CCK-8) assay, wound-healing assay, and transwell invasion assay were conducted to investigate the effects of miR-654-5p on CRC cell proliferation, migration, and invasion, respectively. Moreover, the mechanisms behind miR-654-5p regulates CRC progression were investigated.
Compared with normal cell line, miR-654-5p expression level was significantly suppressed in CRC cells. After overexpression of miR-654-5p, the malignancy behaviors of CRC cells including cell proliferation, migration, and invasion were remarkably decreased. Subsequently, we found hematopoietic cell-specific protein 1-associated protein X-1 (HAX-1) was a putative target for miR-654-5p. Rescue experiments showed overexpression of HAX-1 could partially reversed the effects of miR-654-4p on CRC cell events.
miR-654-5p was reduced expression in CRC cells and could regulate CRC progression via targeting HAX-1.
。miR-654-5p（微小 RNA-654-5p）在癌症中的生物学作用已被先前报道。然而，其在结直肠癌（CRC）中的作用在很大程度上尚不清楚。本研究旨在探讨其在 CRC 中的作用及相关机制。
采用实时定量 PCR（qRT-PCR）检测 miR-654-5p 在 CRC 细胞中的表达模式。细胞计数试剂盒-8（CCK-8）检测、划痕愈合实验和 Transwell 侵袭实验分别用于研究 miR-654-5p 对 CRC 细胞增殖、迁移和侵袭的影响。此外，还研究了 miR-654-5p 调控 CRC 进展的机制。
与正常细胞系相比，CRC 细胞中 miR-654-5p 的表达水平显著下调。过表达 miR-654-5p 后，CRC 细胞的恶性行为，包括细胞增殖、迁移和侵袭能力明显降低。随后，我们发现造血细胞特异性蛋白 1 相关蛋白 X-1（HAX-1）是 miR-654-5p 的一个潜在靶标。挽救实验表明，过表达 HAX-1 可部分逆转 miR-654-4p 对 CRC 细胞事件的影响。
miR-654-5p 在 CRC 细胞中呈低表达，可通过靶向 HAX-1 调节 CRC 进展。
