Developmental & Cell Biology and Center for Complex Biological Systems, UC Irvine, Irvine, CA 92697, USA.
Department of Physics, University of California, San Diego, La Jolla, CA 92093, USA.
Cell Syst. 2020 Mar 25;10(3):287-297.e5. doi: 10.1016/j.cels.2020.02.002. Epub 2020 Feb 26.
The simplest configuration of mitochondria in a cell is as small separate organellar units. Instead, mitochondria often form a dynamic, intricately connected network. A basic understanding of the topological properties of mitochondrial networks, and their influence on cell function is lacking. We performed an extensive quantitative analysis of mitochondrial network topology, extracting mitochondrial networks in 3D from live-cell microscopic images of budding yeast cells. In the presence of fission and fusion, mitochondrial network structures exhibited certain topological properties similar to other real-world spatial networks. Fission and fusion dynamics were required to efficiently distribute mitochondria throughout the cell and generate highly interconnected networks that can facilitate efficient diffusive search processes. Thus, mitochondrial fission and fusion combine to regulate the underlying topology of mitochondrial networks, which may independently impact cell function.
细胞中最简单的线粒体结构是小型独立的细胞器单位。相反，线粒体通常形成一个动态的、错综复杂的连接网络。人们对线粒体网络的拓扑性质及其对细胞功能的影响缺乏基本的了解。我们对线粒体网络拓扑进行了广泛的定量分析，从出芽酵母细胞的活细胞显微镜图像中提取线粒体网络。在有分裂和融合的情况下，线粒体网络结构表现出某些与其他真实空间网络相似的拓扑性质。分裂和融合动力学对于有效地将线粒体分布在整个细胞中并生成高度互联的网络以促进有效的扩散搜索过程是必需的。因此，线粒体的分裂和融合共同调节线粒体网络的基础拓扑结构，这可能独立地影响细胞功能。
