Department of Food Science, The Faculty of Agrobiology, Food and Natural Resources, The Czech University of Life Sciences Prague, 16500 Prague, Czech Republic.
Department of Microbiology, Nutrition and Dietetics, The Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 16500 Prague, Czech Republic.
Nutrients. 2020 Feb 25;12(3):595. doi: 10.3390/nu12030595.
-resveratrol, a well-known plant phenolic compound, has been intensively investigated due to its association with the so-called French paradox. However, despite its high pharmacological potential, -resveratrol has shown relatively low bioavailability. -resveratrol is intensively metabolized in the intestine and liver, yielding metabolites that may be responsible for its high bioactivity. The aim of this study was to investigate and compare the metabolism of -resveratrol (tRes), -resveratrol (cRes) and dihydroresveratrol (dhRes) in an in vitro epithelial model using Caco-2 cell lines. Obtained metabolites of tRes, cRes and dhRes were analyzed by LC/MS Q-TOF, and significant differences in the metabolism of each compound were observed. The majority of tRes was transported unchanged through the Caco-2 cells, while cRes was mostly metabolized. The main metabolite of both and resveratrol observed as a result of colon microbial metabolism, dhRes, was metabolized almost completely, with only traces of the unchanged molecule being found. A sulphate conjugate was identified as the main metabolite of tRes in our model, while a glucuronide conjugate was the major metabolite of cRes and dhRes. Since metabolism of simple phenolics and polyphenols plays a crucial role in their bioavailability, detailed knowledge of their transformation is of high scientific value.
白藜芦醇是一种众所周知的植物酚类化合物，由于与所谓的法国悖论有关，因此受到了广泛研究。然而，尽管白藜芦醇具有很高的药理学潜力，但它的生物利用度相对较低。白藜芦醇在肠道和肝脏中被强烈代谢，产生的代谢物可能是其高生物活性的原因。本研究旨在使用 Caco-2 细胞系研究并比较 -resveratrol（tRes）、-resveratrol（cRes）和二氢白藜芦醇（dhRes）在体外上皮模型中的代谢。通过 LC/MS Q-TOF 分析 tRes、cRes 和 dhRes 的代谢产物，并观察到每种化合物代谢的显著差异。tRes 的大部分未经改变地穿过 Caco-2 细胞运输，而 cRes 则主要被代谢。作为结肠微生物代谢的结果观察到的 和白藜芦醇的主要代谢物 dhRes 几乎完全被代谢，只有痕量的未改变分子被发现。在我们的模型中，鉴定出硫酸盐缀合物是 tRes 的主要代谢物，而葡萄糖醛酸缀合物是 cRes 和 dhRes 的主要代谢物。由于简单酚类和多酚类的代谢在其生物利用度中起着至关重要的作用，因此详细了解它们的转化具有很高的科学价值。
