Mugetti Davide, Pastorino Paolo, Menconi Vasco, Messina Margherita, Masoero Loretta, Ceresa Luisa, Pedron Claudio, Prearo Marino
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, 10154 Torino, Italy.
Dipartimento di Scienze della Vita, Università degli Studi di Trieste, 34127 Trieste, Italy.
Pathogens. 2020 Feb 25;9(3):156. doi: 10.3390/pathogens9030156.
We report the first case of Acipenser iridovirus European (AcIV-E) infection in starry sturgeon ( ) and in sterlet ( ) reared in Northern Italy. During 2018, mortality began in and specimens reared in co-habitation with Russian sturgeon positive for AcIV-E. Molecular analyses were done on the gills to amplify a fragment of the major capsid protein (MCP) gene using real-time PCR against AcIV-E. DNA of the positive samples was further sequenced and phylogenetic analyses were performed. The MCP gene sequences were highly similar to a virus previously identified in Italy (nucleotide identities between 99.38% and 99.69%). Phylogenetic analysis confirmed our hypothesis of passage of the virus from the infected Russian sturgeon. The detection of AcIV-E in new species of the Acipenseridae family may impact on sturgeon production, with relevant economic losses.
我们报告了在意大利北部养殖的俄罗斯鲟（ ）和小体鲟（ ）中首次发现欧洲鲟鱼虹彩病毒（AcIV-E）感染的病例。2018年期间，与感染AcIV-E呈阳性的俄罗斯鲟混养的俄罗斯鲟和小体鲟样本开始出现死亡。对鳃进行了分子分析，使用针对AcIV-E的实时PCR扩增主要衣壳蛋白（MCP）基因的片段。对阳性样本的DNA进行了进一步测序，并进行了系统发育分析。MCP基因序列与之前在意大利鉴定出的一种病毒高度相似（核苷酸同一性在99.38%至99.69%之间）。系统发育分析证实了我们关于病毒从受感染的俄罗斯鲟传播的假设。在鲟科新物种中检测到AcIV-E可能会影响鲟鱼生产，并造成相关经济损失。
