Department of Oral Health Sciences, University of Washington School of Dentistry, Seattle, WA, USA.
Division of Periodontics, Farculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.
BMC Complement Med Ther. 2020 Feb 27;20(1):62. doi: 10.1186/s12906-020-2850-8.
Natural products constitute a promising class of therapeutics for the treatment of gingivitis and periodontitis as well as the maintenance of oral health. However, the limited understanding behind their potential mechanisms and modes of action have hampered their incorporation into popular western therapeutics. This in vitro study characterizes an Ayurvedic herbal extract mixture, which has been clinically shown to promote gingival health and homeostasis.
Telomerase immortalized gingival keratinocytes (TIGK) were infected with either Fusobacterium nucleatum cell wall, live F. nucleatum, IL-1β or TNF-α for 4 hours with and without the herbal extract. The immunomodulatory effects of the extract on host IL-8 production was measured by ELISA.
It was found that the Ayurvedic herbal extract mixture inhibited gingival epithelial cell IL-8 expression in response to both bacterial and host cytokine agonists. The herbal extract inhibited IL-8 stimulated by F. nucleatum cell wall, live F. nucleatum, IL-1β, and TNF-α in a dose-dependent manner that was not a result of host cell death. Furthermore, the extract showed significantly different ID doses demonstrating the differential ability to modulate both stimulated and basal IL-8 levels.
In vitro investigation of this herbal extract mixture revealed that it has the ability to modulate gingival epithelial cell IL-8 expression in response to stimulation by bacterial components and host pro-inflammatory signals. This data demonstrates that the reduction in the gingival epithelial cell IL-8 response may in part be responsible for the previously reported ability of the Ayurvedic herbal extract mixture to reduce gingivitis in two separate human clinical studies.
天然产物是治疗牙龈炎和牙周炎以及维护口腔健康的有前途的治疗药物。然而，由于对其潜在机制和作用方式的了解有限，它们一直未能被纳入流行的西方治疗方法。本体外研究对一种已被临床证实可促进牙龈健康和体内平衡的阿育吠陀草药提取物混合物进行了描述。
用破骨细胞核梭菌细胞壁、活的破骨细胞核梭菌、IL-1β或 TNF-α感染端粒酶永生化牙龈角质形成细胞（TIGK）4 小时，同时或不使用草药提取物。通过 ELISA 测量提取物对宿主 IL-8 产生的免疫调节作用。
发现该阿育吠陀草药提取物混合物可抑制牙龈上皮细胞对细菌和宿主细胞因子激动剂的 IL-8 表达。该草药提取物以剂量依赖性方式抑制破骨细胞核梭菌细胞壁、活的破骨细胞核梭菌、IL-1β和 TNF-α刺激的 IL-8，且这不是宿主细胞死亡的结果。此外，提取物表现出明显不同的 ID 剂量，表明其具有调节刺激和基础 IL-8 水平的不同能力。
对该草药提取物混合物的体外研究表明，它具有调节牙龈上皮细胞对细菌成分和宿主促炎信号刺激的 IL-8 表达的能力。这些数据表明，减少牙龈上皮细胞 IL-8 反应可能部分解释了先前报道的阿育吠陀草药提取物混合物在两项独立的人体临床研究中降低牙龈炎的能力。
