Schueller Katharina, Riva Alessandra, Pfeiffer Stefanie, Berry David, Somoza Veronika
Department of Nutritional and Physiological Chemistry, Faculty of Chemistry, University of ViennaVienna, Austria; Research Network "Chemistry Meets Microbiology", University of ViennaVienna, Austria.
Department of Health Sciences, Università degli Studi di MilanoMilan, Italy; Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, University of ViennaVienna, Austria.
Front Microbiol. 2017 Mar 15;8:416. doi: 10.3389/fmicb.2017.00416. eCollection 2017.
The triggers for the onset of oral diseases are still poorly understood. The aim of this study was to characterize the oral bacterial community in healthy humans and its association with nutrition, oral hygiene habits, and the release of the inflammatory marker IL-8 from gingival epithelial cells (GECs) with and without stimulation by bacterial endotoxins to identify possible indicator operational taxonomic units (OTUs) associated with inflammatory marker status. GECs from 21 healthy participants (13 females, 8 males) were incubated with or without addition of bacterial lipopolysaccharides (LPSs), and the oral microbiota was profiled using 16S rRNA gene-targeted sequencing. The basal IL-8 release after 6 h was between 9.9 and 98.2 pg/ml, and bacterial communities were characteristic for healthy oral microbiota. The composition of the oral microbiota was associated with basal IL-8 levels, the intake of meat, tea, white wine, sweets and the use of chewing gum, as well as flossing habits, allergies, gender and body mass index. Additionally, eight OTUs were associated with high basal levels of IL-8 and GEC response to LPS, with high basal levels of IL-8, and 1 with low basal levels of IL8. The identification of indicator bacteria in healthy subjects with high levels of IL-8 release is of importance as they may be promising early warning indicators for the possible onset of oral diseases.
口腔疾病发病的触发因素仍未得到充分了解。本研究的目的是描述健康人群的口腔细菌群落，及其与营养、口腔卫生习惯，以及在有无细菌内毒素刺激情况下牙龈上皮细胞（GECs）炎症标志物白细胞介素-8（IL-8）释放之间的关联，以确定与炎症标志物状态相关的可能指示性操作分类单元（OTUs）。将来自21名健康参与者（13名女性，8名男性）的GECs在添加或不添加细菌脂多糖（LPSs）的情况下进行培养，并使用靶向16S rRNA基因的测序对口腔微生物群进行分析。6小时后的基础IL-8释放量在9.9至98.2 pg/ml之间，细菌群落具有健康口腔微生物群的特征。口腔微生物群的组成与基础IL-8水平、肉类、茶、白葡萄酒、糖果的摄入量、口香糖的使用，以及使用牙线的习惯、过敏、性别和体重指数有关。此外，8个OTUs与IL-8的高基础水平以及GECs对LPS的反应有关，1个与IL-8的低基础水平有关。在IL-8释放水平高的健康受试者中识别指示细菌很重要，因为它们可能是口腔疾病可能发病的有前景的早期预警指标。
