Departamento de Genética, Instituto de Biociências, UNESP - Univ Estadual Paulista, R. Prof. Dr. Antônio Celso Wagner Zanin s/n, Botucatu, SP CEP 18618-689, Brazil.
Suzano Papel e Celulose SA, Av. Dr. José Lembo 1010, Itapetininga, SP CEP 18207-780, Brazil.
Sci Rep. 2020 Feb 27;10(1):3608. doi: 10.1038/s41598-020-60308-8.
About 95% of Eucalyptus species present an organ known as a lignotuber, a basal woody swelling that holds a large number of dormant buds in a protected position along with carbohydrates and other nutrients. The importance of this trait in Eucalyptus species relates to its regenerative capacity, particularly in the context of coppicing practices and survival in regions of high abiotic stress, especially fire. In this study, we identified and characterized a genomic region associated with the lignotuber trait in commercially important Eucalyptus species by developing a polymorphic marker that co-segregates with lignotuber presence. The marker was then converted into a SCAR (Sequence Characterized Amplified Region) marker, validated in four other Eucalyptus species and hybrids and analyzed in silico. Our investigation presents a marker (ELig) that is effective in identifying individuals with lignotuber. In silico and Southern blot analyses show that the marker is present in a single copy region and is related to auxilin/cyclin-G associated kinase, containing a DnaJ domain. The ELig marker is an important tool that can be used to manage crosses in Eucalyptus breeding programs and inform studies involving lignotuber development and genetics.
大约 95%的桉树物种都有一个称为根瘤的器官，这是一个基部木质的肿胀物，其中包含大量休眠芽，这些芽与碳水化合物和其他营养物质一起被保护在一个位置。这种特性在桉树物种中的重要性与其再生能力有关，特别是在萌芽和在高非生物胁迫地区（特别是火灾）生存的情况下。在这项研究中，我们通过开发一个与根瘤存在共分离的多态性标记，鉴定和表征了商业上重要的桉树物种中的一个与根瘤特性相关的基因组区域。然后，我们将该标记转化为一个 SCAR（序列特征扩增区域）标记，在其他四个桉树物种和杂种中进行验证，并进行了计算机分析。我们的研究提供了一个有效的标记（ELig），用于识别具有根瘤的个体。计算机分析和 Southern blot 分析表明，该标记存在于一个单一拷贝区域，与 auxilin/cyclin-G 相关激酶有关，含有 DnaJ 结构域。ELig 标记是一个重要的工具，可以用于管理桉树育种计划中的杂交，并为涉及根瘤发育和遗传学的研究提供信息。
