ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India.
Animal Genetics & Breeding Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India.
Sci Rep. 2020 Feb 27;10(1):3636. doi: 10.1038/s41598-020-59887-3.
The present study focused upon identification of genome-wide SNPs through the reduced representation approach and to study the genomic divergence of the Indian yak populations. A total of 80 samples belonging to Arunachali yak (N = 20), Himachali yak (N = 20), Ladakhi yak (N = 20) and Sikkimi yak (N = 20) of India were used in the study. The results of the study revealed a total of 579575 high quality SNPs along with 50319 INDELs in the Indian yaks. The observed heterozygosity was found to be high in Himachali yak, followed by Arunachali yak, Ladakhi yak and Sikkimi yaks. The Sikkimi yaks was found to be genetically distant, followed by Ladakhi yaks which was observed to have some few individuals from Arunachali and Himachali yaks. Arunachali and Himachali yaks are found to get clustered together and are genetically similar. The study provides evidence about the genomic diversity in the Indian yak populations and information generated in the present study may help to formulate a suitable breeding plan for endangered Indian yaks. Moreover, the unique yak populations identified in the study will further help to focus attention for future characterization and prioritization of the animals for conservation purposes through the ddRAD approach.
本研究通过简化代表性方法重点鉴定全基因组 SNP，并研究印度牦牛种群的基因组分化。本研究共使用了 80 个样本，包括印度的阿鲁纳恰尔牦牛（N=20）、喜马偕尔牦牛（N=20）、拉达克牦牛（N=20）和锡金牦牛（N=20）。研究结果共发现印度牦牛中的 579575 个高质量 SNP 和 50319 个 INDEL。观察到的杂合度在喜马偕尔牦牛中最高，其次是阿鲁纳恰尔牦牛、拉达克牦牛和锡金牦牛。锡金牦牛被发现遗传距离较远，其次是拉达克牦牛，其中观察到有少数个体来自阿鲁纳恰尔和喜马偕尔牦牛。阿鲁纳恰尔和喜马偕尔牦牛被发现聚集在一起，具有相似的遗传特征。该研究为印度牦牛种群的基因组多样性提供了证据，本研究中生成的信息可能有助于为濒危的印度牦牛制定合适的繁殖计划。此外，研究中鉴定的独特牦牛种群将进一步有助于通过 ddRAD 方法关注未来对动物的特征描述和优先保护。
