Szymańska-Czerwińska Monika, Niemczuk Krzysztof, Galińska Elżbieta Monika
Laboratory of Serological Diagnosis /Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Puławy, Poland.
Department of Allergy and Environmental Health, Institute of Rural Health, Lublin, Poland.
Ann Agric Environ Med. 2013;20(4):682-6.
Chlamydia spp. is an obligate intracellular agent that causes chlamydiosis in animals and humans. The aim of the presented study was to investigate the prevalence of Chlamydia infection in the Polish cattle population, both asymptomatic and having reproductive disorders. The study was performed on 4,475 serum samples collected from 16 Polish provinces at the turn of 2009-2011. The samples (3,419 from asymptomatic cattle and 1,056 from cattle with reproductive disorders) were tested by complement fixation test (CFT). Moreover, 160 and 201 samples of biological materials from both groups of cattle, respectively, were tested by nested PCR. The results obtained for two tested groups were compared by χ2 (ch-squared) test, both individually for each region (province), and generally for the whole country. The CFT results showed that the seroprevalence of Chlamydia spp. infections in the asymptomatic cattle population was 4.15%, while in the cattle with reproductive disorders--7.20%. There was a significant statistical difference between compared groups for whole country, but there were no significant differences for individual provinces. The results of PCR showed that Chlamydia spp. was present in both asymptomatic cattle and cattle having reproductive disorders. The nested PCR study confirmed the presence of Chlamydia abortus and Chlamydia suis in the tested samples. The presented study indicates that infections with Chlamydia spp. are present among Polish cattle, but the percentage of infected animals is not high.
衣原体属是一种专性细胞内病原体，可导致动物和人类感染衣原体病。本研究的目的是调查波兰牛群中衣原体感染的流行情况，包括无症状感染和患有生殖系统疾病的牛群。该研究对2009 - 2011年之交从波兰16个省份采集的4475份血清样本进行。样本（3419份来自无症状牛，1056份来自患有生殖系统疾病的牛）通过补体结合试验（CFT）进行检测。此外，分别对两组牛的160份和201份生物材料样本进行巢式PCR检测。通过χ²（卡方）检验对两个检测组的结果进行比较，分别针对每个地区（省份）单独比较，以及对整个国家进行总体比较。CFT结果显示，无症状牛群中衣原体属感染的血清阳性率为4.15%，而患有生殖系统疾病的牛群中为7.20%。全国范围内比较组之间存在显著统计学差异，但各省份之间无显著差异。PCR结果表明，衣原体属在无症状牛和患有生殖系统疾病的牛中均有存在。巢式PCR研究证实检测样本中存在流产衣原体和猪衣原体。本研究表明，波兰牛群中存在衣原体属感染，但感染动物的百分比不高。
