Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA; email:
Annu Rev Anal Chem (Palo Alto Calif). 2020 Jun 12;13(1):363-380. doi: 10.1146/annurev-anchem-061318-115009. Epub 2020 Feb 28.
Protein separations have gained increasing interest over the past two decades owing to the dramatic growth of proteins as therapeutics and the completion of the Human Genome Project. About every decade, the field of protein high-performance liquid chromatography (HPLC) seems to mature, having reached what appears to be a theoretical limit. But then scientists well versed in the basic principles of HPLC invented a way around the limit, generating another decade of exciting progress. There is still the need for higher resolution and better compatibility with mass spectrometry because it is an essential tool for identification of proteins and their modifications. To make advances, the fundamental principles need to be understood. This review covers recent advances and current needs in the context of the principles that underlie the many contributions to peak broadening.
在过去的二十年中，由于蛋白质作为治疗药物的急剧增长和人类基因组计划的完成，蛋白质分离技术引起了越来越多的关注。大约每十年，蛋白质高效液相色谱（HPLC）领域似乎就会成熟一次，达到似乎是理论极限的程度。但是，精通 HPLC 基本原理的科学家们找到了突破这一极限的方法，从而又取得了十年的令人兴奋的进展。仍然需要更高的分辨率和更好的与质谱法的兼容性，因为它是鉴定蛋白质及其修饰物的重要工具。为了取得进展，需要理解基本原理。本综述涵盖了在许多导致峰展宽的贡献所依据的原理的背景下的最新进展和当前需求。
