Department of Molecular Medicine and Neurobiology, The Scripps Research Institute, La Jolla, California 92037, USA; email:
Department of Biosciences and Technology of Bioactive Products, Institute of Biology, University of Campinas, São Paulo 13083-862, Brazil.
Annu Rev Anal Chem (Palo Alto Calif). 2020 Jun 12;13(1):315-336. doi: 10.1146/annurev-anchem-091619-093003. Epub 2020 Feb 28.
Technological advances in mass spectrometry have enabled the extensive identification, characterization, and quantification of proteins in any biological system. In disease processes proteins are often altered in response to external stimuli; therefore, proteomics, the large-scale study of proteins and their functions, represents an invaluable tool for understanding the molecular basis of disease. This review highlights the use of mass spectrometry-based proteomics to study the pathogenesis, etiology, and pathology of several neglected tropical diseases (NTDs), a diverse group of disabling diseases primarily associated with poverty in tropical and subtropical regions of the world. While numerous NTDs have been the subject of proteomic studies, this review focuses on Buruli ulcer, dengue, leishmaniasis, and snakebite envenoming. The proteomic studies highlighted provide substantial information on the pathogenic mechanisms driving these diseases; they also identify molecular targets for drug discovery and development and uncover promising biomarkers that can assist in early diagnosis.
质谱技术的进步使人们能够广泛地鉴定、描述和定量分析任何生物系统中的蛋白质。在疾病过程中，蛋白质常常会因外部刺激而发生改变；因此，蛋白质组学作为一种大规模研究蛋白质及其功能的方法，是理解疾病分子基础的宝贵工具。本文重点介绍了基于质谱的蛋白质组学在研究几种被忽视热带病（NTDs）的发病机制、病因和病理学中的应用，这些疾病是一组主要与世界热带和亚热带地区贫困相关的致残疾病。虽然许多 NTD 都已经成为蛋白质组学研究的对象，但本文重点介绍了布鲁里溃疡、登革热、利什曼病和蛇咬伤中毒。所强调的蛋白质组学研究为驱动这些疾病的致病机制提供了大量信息；它们还确定了药物发现和开发的分子靶点，并揭示了有希望的生物标志物，可用于早期诊断。
