Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, China; National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Huaiyin Institute of Technology, Huai'an 223003, China; Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China.
Department of Geriatric Medicine, Huai'an Second People's Hospital, Huai'an 223002, Jiangsu, China.
Int J Biol Macromol. 2020 Jun 1;152:314-326. doi: 10.1016/j.ijbiomac.2020.02.243. Epub 2020 Feb 25.
The aim of this paper was to investigate the extraction, purification and characterization of sulphated polysaccharide (BQPS) from Bellamya quadrata and its stabilization roles on atherosclerotic plaque. Firstly, crude polysaccharide (CBQP) from Bellamya quadrata was extracted by protease enzyme assisted extraction. Moreover, its optimal parameters were obtained by the response surface method as follows: the ratio of water to raw material of 24:1, enzyme dosage of 285 U/g, enzymolysis pH value of 4.7 and temperature of 67 °C. Secondly, CBQP was further purified to obtain the target polysaccharide BQPS by Q Sepharose Fast Flow and Sephacryl S-400 gel column chromatography. Then, the characterization of BQPS revealed that it possessed a total polysaccharide content of 91.88 ± 1.23%, sulfuric acid group content of 9.12 ± 1.59% and molecular weight of 91.1 kDa. BQPS was only consisted of glucose without any proteins. Finally, BQPS was confirmed to have a significant stabilizing effect on atherosclerotic plaque and its mechanism was related to the selective promotion of autophagy with the precisely right strength.
本文旨在研究从四角蛤蜊中提取、纯化和表征硫酸多糖（BQPS）及其对动脉粥样硬化斑块的稳定作用。首先，通过蛋白酶酶辅助提取从四角蛤蜊中提取粗多糖（CBQP）。此外，通过响应面法获得其最佳参数如下：水与原料的比例为 24:1，酶用量为 285 U/g，酶解 pH 值为 4.7，温度为 67°C。其次，通过 Q Sepharose Fast Flow 和 Sephacryl S-400 凝胶柱层析进一步纯化 CBQP 以获得目标多糖 BQPS。然后，BQPS 的特性表明，它的总多糖含量为 91.88±1.23%，硫酸基团含量为 9.12±1.59%，分子量为 91.1 kDa。BQPS 仅由葡萄糖组成，不含任何蛋白质。最后，BQPS 被证实对动脉粥样硬化斑块具有显著的稳定作用，其作用机制与选择性促进自噬有关，且具有恰到好处的强度。
