Fisheries College, Henan Normal University, Xinxiang 453007, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Genomics. 2020 May;112(3):2603-2614. doi: 10.1016/j.ygeno.2020.01.026. Epub 2020 Feb 25.
Amur catfish is extensively distributed and cultured in Asian countries. Despite of economic importance, the genomic information of this species remains limited. A reference transcriptome of Amur catfish was assembled and the sex-biased gene expression in the gonads was characterized using RNA-sequencing. The assembled transcriptome of Amur catfish consisted of 74,840 transcripts. The N50, mean length and max length of transcripts are 1970, 1235 and 16,748 bp. Putative sex-specific transcripts were identified and sex-specific expression of the representative genes was verified by RT-PCR. Differential expression analysis identified 5401 ovary-biased and 5618 testis-biased genes. The ovary-biased genes were mainly enriched in pathways such as RNA transport and ribosome biogenesis in eukaryotes. The testis-biased genes were enriched in calcium signaling and cytokine-cytokine receptor interaction, etc. Our data provide a valuable genomic resource for further investigating the genetic basis of sex determination, sex differentiation and sexual dimorphism of catfish.
虹鳟鱼分布广泛，在亚洲国家广泛养殖。尽管具有经济重要性，但该物种的基因组信息仍然有限。本研究组装了虹鳟鱼的参考转录组，并利用 RNA 测序对其性腺中的性别偏向基因表达进行了研究。组装的虹鳟鱼转录组由 74840 个转录本组成。转录本的 N50、平均长度和最大长度分别为 1970bp、1235bp 和 16748bp。鉴定了性别特异性转录本，并通过 RT-PCR 验证了代表性基因的性别特异性表达。差异表达分析鉴定了 5401 个卵巢偏向和 5618 个睾丸偏向基因。卵巢偏向基因主要富集在真核生物的 RNA 转运和核糖体生物发生等途径中。睾丸偏向基因富集在钙信号和细胞因子-细胞因子受体相互作用等途径中。我们的数据为进一步研究鱼类性别决定、性别分化和性二型性的遗传基础提供了有价值的基因组资源。
