Engineering Research Center of Mine Ecological Construction, Ministry of Education, China University of Mining and Technology, Xuzhou 221116, China.
Engineering Research Center of Mine Ecological Construction, Ministry of Education, China University of Mining and Technology, Xuzhou 221116, China.
J Environ Sci (China). 2021 May;103:108-118. doi: 10.1016/j.jes.2020.10.002. Epub 2020 Nov 6.
Biotrickling filters (BTFs) for hydrophobic chlorobenzene (CB) purification are limited by mass transfer and biodegradation. The CB mass transfer rate could be improved by 150 mg/L rhamnolipids. This study evaluated the combined use of Fe and Zn to enhance biodegradation in a BTF over 35 day. The effects of these trace elements were analysed under different inlet concentrations (250, 600, 900, and 1200 mg/L) and empty bed residence times (EBRTs; 60, 45, and 32 sec). Batch experiments showed that the promoting effects of Fe/Zn on microbial growth and metabolism were highest for 3 mg/L Fe and 2 mg/L Zn, followed by 2 mg/L Zn, and lowest at 3 mg/L Fe. Compared to BTF in the absence of Fe and Zn, the average CB elimination capacity and removal efficiency in the presence of Fe and Zn increased from 61.54 to 65.79 g/(m⋅hr) and from 80.93% to 89.37%, respectively, at an EBRT of 60 sec. The average removal efficiency at EBRTs of 60, 45, and 32 sec increased by 2.89%, 5.63%, and 11.61%, respectively. The chemical composition (proteins (PN), polysaccharides (PS)) and functional groups of the biofilm were analysed at 60, 81, and 95 day. Fe and Zn significantly enhanced PN and PS secretion, which may have promoted CB adsorption and biodegradation. High-throughput sequencing revealed the promoting effect of Fe and Zn on bacterial populations. The combination of Fe and Zn with rhamnolipids was an efficient method for improving CB biodegradation in BTFs.
生物滴滤塔（BTFs）在净化疏水性氯苯（CB）时受到传质和生物降解的限制。通过添加 150mg/L 鼠李糖脂可以提高 CB 的传质速率。本研究评估了在 35 天内使用铁和锌来增强 BTF 中的生物降解的联合作用。在不同的入口浓度（250、600、900 和 1200mg/L）和空床停留时间（EBRT；60、45 和 32 秒）下分析了这些微量元素的影响。批处理实验表明，铁/锌对微生物生长和代谢的促进作用以 3mg/L 铁和 2mg/L 锌最高，其次是 2mg/L 锌，而 3mg/L 铁最低。与不存在铁和锌的 BTF 相比，在存在铁和锌的情况下，EBRT 为 60 秒时 CB 的平均消除容量和去除效率分别从 61.54 增加到 65.79g/（m·hr）和从 80.93%增加到 89.37%。在 EBRTs 为 60、45 和 32 秒时，平均去除效率分别提高了 2.89%、5.63%和 11.61%。在第 60、81 和 95 天分析了生物膜的化学组成（蛋白质（PN）、多糖（PS））和功能基团。铁和锌显著增强了 PN 和 PS 的分泌，这可能促进了 CB 的吸附和生物降解。高通量测序揭示了铁和锌对细菌种群的促进作用。铁和锌与鼠李糖脂的结合是提高 BTF 中 CB 生物降解的有效方法。
