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用于：开发、表征及跨物种扩增的微卫星标记

Microsatellite markers for : Development, characterization, and cross-species amplification.

作者信息

Vít Petr, Krak Karol, Douda Jan, Mandák Bohumil

机构信息

Faculty of Environmental Sciences Czech University of Life Sciences Prague Kamýcká 129 Praha 6-Suchdol CZ-165 00 Czech Republic.

The Czech Academy of Sciences Institute of Botany Zámek 1 Průhonice CZ-252 43 Czech Republic.

出版信息

Appl Plant Sci. 2020 Feb 14;8(2):e11323. doi: 10.1002/aps3.11323. eCollection 2020 Feb.

DOI:10.1002/aps3.11323
PMID:32110503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7035427/
Abstract

PREMISE

A set of polymorphic nuclear microsatellite loci was developed and tested for use in population genetic analyses of (Agavaceae) and related species.

METHODS AND RESULTS

Sequences of 110 primers were extracted in silico from Illumina MiSeq genome skimming data. The degree of polymorphism of 19 loci was tested in four . populations collected in Central and Eastern Europe. The average number of alleles per loci ranged from two to 17, and levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.100 to 0.900, respectively. Primers were successfully amplified in the closely related species (12 loci) and (six loci), whereas they mostly failed to amplify in the phylogenetically more-distant species (three loci) and . (no amplification).

CONCLUSIONS

This newly developed set of polymorphic nuclear microsatellite markers will be useful for population genetic investigation of . and closely related species.

摘要

前提

开发并测试了一组多态性核微卫星位点，用于龙舌兰科及相关物种的群体遗传分析。

方法与结果

从Illumina MiSeq基因组浅层测序数据中通过计算机模拟提取了110个引物的序列。在从中欧和东欧采集的4个龙舌兰科种群中测试了19个位点的多态性程度。每个位点的平均等位基因数从2到17不等，观察到的杂合度水平和预期杂合度水平分别从0.000到1.000和从0.100到0.900不等。引物在近缘物种狭叶龙舌兰（12个位点）和小花龙舌兰（6个位点）中成功扩增，而在系统发育关系较远的物种大刺龙舌兰（3个位点）和鬼脚掌（无扩增）中大多未能扩增。

结论

这组新开发的多态性核微卫星标记将有助于龙舌兰科及近缘物种的群体遗传研究。