十字花科植物及其近缘物种微卫星标记的特征分析
Characterization of microsatellite markers for (Brassicaceae) and related species.
作者信息
Cuenot Yves, Gómez José M, González-Megias Adela, Pannell John R, Torices Rubén
机构信息
Department of Ecology and Evolution Université de Lausanne CH-1015 Lausanne Switzerland.
Estación Experimental de Zonas Áridas Consejo Superior de Investigaciones Científicas Ctra. de Sacramento s/n, La Cañada de San Urbano E-04120 Almería Spain.
出版信息
Appl Plant Sci. 2018 Aug 21;6(8):e01172. doi: 10.1002/aps3.1172. eCollection 2018 Aug.
PREMISE OF THE STUDY
Polymorphic microsatellite markers were developed to study population structure and mating patterns of the monocarpic herb (Brassicaceae).
METHODS AND RESULTS
Illumina MiSeq sequencing was used to develop a panel of 15 polymorphic microsatellite markers that were tested across 77 individuals from three populations on the Iberian Peninsula. All markers were polymorphic in at least two studied populations, and the number of alleles ranged from one to 11 per locus. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.153 to 0.865, respectively. Nine and 11 loci were successfully amplified in the congeneric species and , respectively.
CONCLUSIONS
The 15 microsatellite markers will be useful for population genetic studies of the genus . These markers will serve as a useful tool for exploring population structure and mating patterns of .
研究前提
开发多态性微卫星标记以研究单果草本植物（十字花科）的种群结构和交配模式。
方法与结果
使用Illumina MiSeq测序开发了一组15个多态性微卫星标记，并在伊比利亚半岛三个种群的77个个体中进行了测试。所有标记在至少两个研究种群中具有多态性，每个位点的等位基因数从1到11不等。观察到的杂合度和预期杂合度水平分别为0.000至1.000和0.153至0.865。分别在同属物种和中成功扩增了9个和11个位点。
结论
这15个微卫星标记将有助于该属的种群遗传学研究。这些标记将作为探索种群结构和交配模式的有用工具。
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