Fatehi-Pouladi Soheil, Anderson Bruce C, Wootton Brent, Wallace Sarah J, Bissegger Sonja, Rozema Lloyd, Weber Kela P
Golder Associates Ltd., 1931 Robertson Road, Ottawa, ON K2H 5B7, Canada.
Department of Civil Engineering, Queen's University, 58 University Ave., Kingston, ON K7L 3N6, Canada.
Plants (Basel). 2020 Feb 26;9(3):289. doi: 10.3390/plants9030289.
The microbial characteristics of four vegetated and one unplanted wood-chip bioreactors treating greenhouse effluent were investigated in a continuous experiment operated for over 2.5 years. The bioreactors were designed to reduce nitrate concentrations via naturally induced microbial denitrification. The vegetation type and reactor depth were both found to be significant factors in defining the mixed microbial activity. However, a consistent correlation between the abundance of the denitrifying communities and reactor depth could not be found across all reactors. The media samples from the unit planted with displayed higher microbial activities compared with the other reactors. This plant's root-associated bacteria also demonstrated the greatest copies of the denitrifying genes and . The most abundant denitrifier communities and those encoding the gene were found in the unplanted reactor, followed by the a unit. The reactor demonstrated greater microbial activity and denitrification capacity at the depth of 20 cm, while the greatest denitrification capacity in the unplanted reactor was found at the depth of 60 cm. These findings indicated the importance of the rhizosphere to support microbial community establishment and growth in the vicinity of the plant's roots, although those populations may eventually develop in an unplanted environment.
在一项持续运行超过2.5年的连续实验中，研究了四个种植植物和一个未种植植物的木屑生物反应器处理温室废水的微生物特性。这些生物反应器旨在通过自然诱导的微生物反硝化作用降低硝酸盐浓度。植被类型和反应器深度均被发现是定义混合微生物活性的重要因素。然而，在所有反应器中，反硝化群落丰度与反应器深度之间并未发现一致的相关性。与其他反应器相比，种植了[具体植物名称未给出]的单元的介质样本显示出更高的微生物活性。该植物的根际相关细菌也显示出反硝化基因[具体基因名称未给出]和[具体基因名称未给出]的最大拷贝数。在未种植植物的反应器中发现了最丰富的反硝化群落以及编码[具体基因名称未给出]基因的群落，其次是[具体单元名称未给出]单元。[具体反应器名称未给出]反应器在20厘米深度处表现出更大的微生物活性和反硝化能力，而未种植植物的反应器在60厘米深度处发现了最大的反硝化能力。这些发现表明了[具体植物名称未给出]根际对于支持植物根系附近微生物群落的建立和生长的重要性，尽管这些种群最终可能在未种植植物的环境中发展。
