Gupta Ravi, Min Cheol Woo, Kim So Wun, Yoo Ju Soon, Moon Ah-Ram, Shin Ah-Young, Kwon Suk-Yoon, Kim Sun Tae
Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India.
Department of Plant Bioscience, Pusan National University, Miryang 627-707, Korea.
Plants (Basel). 2020 Feb 26;9(3):290. doi: 10.3390/plants9030290.
Tomato spotted wilt virus (TSWV), transmitted by small insects known as thrips, is one of the major threats to tomato productivity across the globe. In addition to tomato, this virus infects more than 1000 other plants belonging to 85 families and is a cause of serious concern. Very little, however, is known about the molecular mechanism of TSWV induced signaling in plants. Here, we used a tandem mass tags (TMT)-based quantitative proteome approach to investigate the protein profiles of tomato leaves of two cultivars (cv 2621 and 2689; susceptible and resistant to TSWV infection, respectively) following TSWV inoculation. This approach resulted in the identification of 5112 proteins of which 1022 showed significant changes in response to TSWV. While the proteome of resistant cultivar majorly remains unaltered, the proteome of susceptible cultivar showed distinct differences following TSWV inoculation. TSWV modulated proteins in tomato included those with functions previously implicated in plant defense including secondary metabolism, reactive oxygen species (ROS) detoxification, mitogen-activated protein (MAP) kinase signaling, calcium signaling and jasmonate biosynthesis, among others. Taken together, results reported here provide new insights into the TSWV induced signaling in tomato leaves and may be useful in the future to manage this deadly disease of plants.
番茄斑萎病毒（TSWV）通过名为蓟马的小昆虫传播，是全球番茄生产力面临的主要威胁之一。除了番茄，这种病毒还感染属于85个科的1000多种其他植物，令人严重担忧。然而，关于TSWV在植物中诱导信号传导的分子机制知之甚少。在这里，我们使用基于串联质量标签（TMT）的定量蛋白质组学方法，研究了接种TSWV后两个番茄品种（cv 2621和2689，分别对TSWV感染敏感和抗性）的叶片蛋白质谱。这种方法鉴定出了5112种蛋白质，其中1022种在对TSWV的反应中表现出显著变化。虽然抗性品种的蛋白质组基本保持不变，但敏感品种的蛋白质组在接种TSWV后表现出明显差异。番茄中TSWV调节的蛋白质包括那些先前与植物防御有关的功能蛋白，包括次生代谢、活性氧（ROS）解毒、丝裂原活化蛋白（MAP）激酶信号传导、钙信号传导和茉莉酸生物合成等。综上所述，本文报道的结果为TSWV在番茄叶片中诱导的信号传导提供了新的见解，未来可能有助于管理这种致命的植物病害。
