Phytopathology. 2003 Oct;93(10):1223-7. doi: 10.1094/PHYTO.2003.93.10.1223.
ABSTRACT Spread of Tomato spotted wilt virus (TSWV) and population development of its vector Frankliniella occidentalis were studied on the pepper accessions CPRO-1 and Pikante Reuzen, which are resistant and susceptible to thrips, respectively. Viruliferous thrips were released on plants of each accession (nonchoice tests) or on plants in a 1:1 mixture of both accessions (choice tests) in small cages containing 8 or 16 plants. Significantly fewer CPRO-1 plants became infected in the primary infection phase in both tests. In the nonchoice test, virus infection of the resistant plants did not increase after the initial infection, but all plants eventually became infected when mixtures of both cultivars were challenged in the secondary infection phase. Secondary spread of TSWV from an infected resistant or susceptible source plant was significantly slower to resistant plants than to susceptible plants, independent of source plant phenotype. The restricted introduction and spread of TSWV in the thrips-resistant cultivar was confirmed in a large-scale greenhouse experiment. The restricted and delayed TSWV spread to plants of the resistant accession in both the cage and the greenhouse experiment was explained by impeded thrips population development. The results obtained indicate that thrips resistance may provide a significant protection to TSWV infection, even when the crop is fully susceptible to the virus.
摘要 在对分别对具有抗虫和感虫特性的辣椒品种 CPRO-1 和 Pikante Reuzen 进行的研究中，我们调查了番茄斑萎病毒（TSWV）的传播以及其媒介烟粉虱种群的发展情况。将带毒烟粉虱释放到每一个品种的植株（非选择测试）或在两个品种各占一半的植株混合物（选择测试）的小笼子里的 8 或 16 株植物上。在这两项测试中，CPRO-1 植株在原发性感染阶段的感染数量明显减少。在非选择测试中，在初始感染后，抗虫植株的病毒感染没有增加，但当在二次感染阶段对两个品种的混合物进行挑战时，所有植株最终都被感染。从受感染的抗性或敏感源植物传播的 TSWV 的二次传播对抗性植物的传播速度明显比敏感植物慢，而与源植物的表型无关。在大规模温室实验中，证实了 TSWV 在抗虫品种中的受限引入和传播。在笼和温室实验中，TSWV 向抗性品种植物的传播受限和延迟是由烟粉虱种群发育受阻所导致的。研究结果表明，即使作物对病毒完全易感，抗虫性也可能为 TSWV 感染提供显著的保护。
