Department of Animal genetics and breeding, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, Henan, China.
Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, Henan, China.
BMC Genet. 2020 Feb 28;21(1):22. doi: 10.1186/s12863-020-0831-z.
The SH3RF2 gene is a protein-coding gene located in a quantitative trait locus associated with body weight, and its deletion has been shown to be positively associated with body weight in chickens.
In the present study, CNV in the SH3RF2 gene was detected in 4079 individuals from 17 populations, including the "Gushi ×Anka" F2 resource population and populations of Chinese native chickens, commercial layers, and commercial broilers. The F2 resource population was then used to investigate the genetic effects of the chicken SH3RF2 gene. The results showed that the local chickens and commercial layers were all homozygous for the wild-type allele. Deletion mutation individuals were detected in all of the commercial broiler breeds except Hubbard broiler. A total of, 798 individuals in the F2 resource group were used to analyze the effects of genotype (DD/ID/II) on chicken production traits. The results showed that CNV was associated with 2-, 6-, 10-, and 12-week body weight (P = 0.026, 0.042, 0.021 and 0.039 respectively) and significantly associated with 8-week breast bone length (P = 0.045). The mutation was significantly associated with 8-week body weight (P = 0.007) and 4-week breast bone length (P = 0.010). CNV was significantly associated with evisceration weight, leg muscle weight, carcass weight, breast muscle weight and gizzard weight (P = 0.032, 0.033, 0.045, 0.004 and 0.000, respectively).
CNV of the SH3RF2 gene contributed to variation in the growth and weight gain of chickens.
SH3RF2 基因是一个位于与体重相关的数量性状基因座上的蛋白质编码基因，其缺失已被证明与鸡的体重呈正相关。
本研究在包括“固始鸡×安康鸡”F2 资源群体和中国地方鸡种、商品蛋鸡、商品肉鸡群体在内的 17 个群体的 4079 个个体中检测到 SH3RF2 基因的 CNV。然后利用 F2 资源群体研究鸡 SH3RF2 基因的遗传效应。结果表明，地方鸡种和商品蛋鸡均为野生型等位基因纯合子。除哈伯德肉鸡外，所有商品肉鸡品种均检测到缺失突变个体。利用 F2 资源群体中的 798 个个体分析基因型（DD/ID/II）对鸡生产性状的影响。结果表明，CNV 与 2、6、10 和 12 周体重（P = 0.026、0.042、0.021 和 0.039）显著相关，与 8 周胸骨长度显著相关（P = 0.045）。突变与 8 周体重（P = 0.007）和 4 周胸骨长度（P = 0.010）显著相关。CNV 与屠宰体重、腿肌重、胴体重、胸肌重和肌胃重显著相关（P = 0.032、0.033、0.045、0.004 和 0.000）。
SH3RF2 基因的 CNV 导致鸡的生长和体重增加发生变异。
