USDA-APHIS-PPQ-Science & Technology, Edinburg, TX.
Royal Museum for Central Africa, Tervuren, Belgium.
J Econ Entomol. 2020 Jun 6;113(3):1455-1470. doi: 10.1093/jee/toaa024.
The Mediterranean fruit fly, Ceratitis capitata (Weidemann), is one of the most economically important tephritid species worldwide. It has spread across six geographic regions as a result of successful invasions and continues to cause substantial losses to agricultural communities. Our study examined 1,864 flies originating from 150 localities, using mitochondrial DNA sequencing methods. We tested for population structure and revealed the genetic diversity for 1,592 specimens gathered from 144 wild fly collections from 46 countries representing the entire geographic range for this species. We also include in this study 272 Sterile Insect Technique (SIT) specimens from four SIT facilities. We recovered 202 haplotypes from the current sampling and updated previously published work to reveal a total of 231 haplotypes for this pest. These data show population structure at and below the regional level for these collections, shedding light on the current demographics for this species. We observed four common haplotypes, seen among 62% of the samples sequenced that have worldwide distribution. Three haplotypes were seen in SIT flies, with one seen as the predominant haplotype. Our work showed that two of the haplotypes were private to SIT flies, not present among wild fly collections. However, a third haplotype common among wild fly collections was also seen in one SIT facility but at a low frequency based on the current sampling. We provide guidance on the interpretation of these methods for the source estimation of current and future infestations.
地中海实蝇，Ceratitis capitata (Weidemann)，是世界上最重要的经济重要的实蝇物种之一。由于成功的入侵，它已经扩散到六个地理区域，并继续对农业社区造成巨大的损失。我们的研究使用线粒体 DNA 测序方法检查了 1864 只来自 150 个地点的苍蝇。我们测试了种群结构，并揭示了从 46 个国家的 144 个野生蝇收集地采集的 1592 个标本的遗传多样性，这些国家代表了该物种的整个地理范围。我们还包括了这项研究中的 272 只来自四个不育昆虫技术 (SIT) 设施的 SIT 标本。我们从当前的采样中恢复了 202 个单倍型，并更新了以前发表的工作，以揭示这种害虫的总共 231 个单倍型。这些数据显示了这些收集物的区域和区域以下的种群结构，揭示了该物种的当前人口统计学情况。我们观察到了四种常见的单倍型，在测序的 62%的样本中都有分布，这些样本分布在世界各地。在 SIT 蝇中观察到了三个单倍型，其中一个是主要的单倍型。我们的工作表明，两种单倍型是 SIT 蝇所特有的，在野生蝇收集物中不存在。然而，在一个 SIT 设施中也观察到了一种在野生蝇收集物中常见的单倍型，但根据目前的采样，其频率较低。我们提供了关于这些方法的解释，以用于当前和未来疫情的来源估计。
