利用生物工程生物发光技术在体内追踪干细胞移植。
Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo.
机构信息
Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.
Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
出版信息
Methods Mol Biol. 2020;2126:1-11. doi: 10.1007/978-1-0716-0364-2_1.
Abstract
Bioluminescence imaging enables the real-time detection and tracking of engrafted cells in vivo noninvasively and dynamically. By detecting and quantifying the photons released from the oxidation of luciferin catalyzed by luciferase enzymes, this approach has proven effective in tracking engrafted stem cell survival and retention, making it a powerful tool to monitor cell fate after transplantation without animal sacrifice. Here we describe a protocol that allows luciferase-labeled stem cells to be imaged and tracked in vivo by bioluminescent imaging via an IVIS spectrum imaging system.
摘要
生物发光成像是一种非侵入性和动态的方法，可以实时检测和跟踪体内移植的细胞。通过检测和量化荧光素酶催化下荧光素氧化释放的光子，该方法已被证明可有效跟踪移植干细胞的存活和保留，是一种无需牺牲动物即可监测移植后细胞命运的强大工具。在这里，我们描述了一种通过 IVIS 光谱成像系统进行生物发光成像来对标记有荧光素酶的干细胞进行体内成像和跟踪的方案。
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