利用天然发光酶追踪特定细胞群体。

Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations.

机构信息

Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.

Nanotechnology Innovation Center of Kansas State, Kansas State University, Manhattan, KS, USA.

出版信息

Methods Mol Biol. 2020;2126:13-20. doi: 10.1007/978-1-0716-0364-2_2.

DOI:10.1007/978-1-0716-0364-2_2

PMID:32112375

Abstract

Luminol-based bioluminescence imaging allows noninvasive tracking of oxidatively active cells such as neutrophils. Luminol is given intravenously or intraperitoneally, followed by bioluminescence imaging at 425 nm. Here we describe a method for tracking neutrophil extravasation into an inflammatory site, especially focusing on mammary carcinoma.

摘要

鲁米诺（luminol）基生物发光成像是一种非侵入性的方法，可以追踪氧化活性细胞，如中性粒细胞。将鲁米诺经静脉内或腹腔内给药，然后在 425nm 处进行生物发光成像。在这里，我们描述了一种跟踪中性粒细胞渗出到炎症部位的方法，特别是特别关注乳腺癌。