Department of Anatomy and Physiology, 228 Coles Hall, Kansas State University, Manhattan, KS 66506, USA.
J Photochem Photobiol B. 2013 Oct 5;127:223-8. doi: 10.1016/j.jphotobiol.2013.08.017. Epub 2013 Sep 8.
Polymorphonuclear neutrophils (PMNs) are the most abundant circulating blood leukocytes. They are part of the innate immune system and provide a first line of defense by migrating toward areas of inflammation in response to chemical signals released from the site. Some solid tumors, such as breast cancer, also cause recruitment and activation of PMNs and release of myeloperoxidase. In this study, we demonstrate that administration of luminol to mice that have been transplanted with 4T1 mammary tumor cells permits the detection of myeloperoxidase activity, and consequently, the location of the tumor. Luminol allowed detection of activated PMNs only two days after cancer cell transplantation, even though tumors were not yet palpable. In conclusion, luminol-bioluminescence imaging (BLI) can provide a pathway towards detection of solid tumors at an early stage in preclinical tumor models.
多形核粒细胞（PMN）是循环血液白细胞中最丰富的细胞。它们是先天免疫系统的一部分，通过向炎症部位迁移来响应从该部位释放的化学信号，提供第一道防线。一些实体瘤，如乳腺癌，也会导致 PMN 的募集和激活，并释放髓过氧化物酶。在这项研究中，我们证明给已经移植了 4T1 乳腺肿瘤细胞的小鼠施用鲁米诺，允许检测髓过氧化物酶活性，从而检测肿瘤的位置。在肿瘤还不可触及的情况下，在癌细胞移植后仅两天，鲁米诺就可以检测到活化的 PMN。总之，鲁米诺-生物发光成像（BLI）可以为临床前肿瘤模型中早期检测实体瘤提供一种途径。
