磁共振成像技术在单细胞研究中的应用

Magnetic Resonance Imaging of Single Cells.

机构信息

Department of Chemistry, Kansas State University, Manhattan, KS, USA.

Department of Chemistry and Johnson Cancer Center, Kansas State University, Manhattan, KS, USA.

出版信息

Methods Mol Biol. 2020;2126:95-106. doi: 10.1007/978-1-0716-0364-2_9.

DOI:10.1007/978-1-0716-0364-2_9

PMID:32112382

Abstract

This chapter discusses a methodology for simultaneously imaging stem cells and endothelial cells within polysaccharide-based scaffolds for tissue engineering. These scaffolds were then implanted into nude mice. Human mesenchymal stem cells (HMSCs) were labeled with the T-marker Gd(III)-DOTAGA-functionalized polysiloxane nanoparticles (GdNPs), whereas endothelial umbilical vein cells (HUVECs) were labeled with citrate-stabilized maghemite nanoparticles (IONPs), which predominantly shorten the T-relaxation times of the water molecules in scaffolds and tissue. Dual cell detection was achieved by performing T- and T-weighted MRI in both tissue scaffolds and in vivo.

摘要

本章讨论了一种用于同时对组织工程中多糖基支架内的干细胞和内皮细胞进行成像的方法。然后将这些支架植入裸鼠体内。人骨髓间充质干细胞（HMSCs）用 T 标志物 Gd（III）-DOTAGA 功能化的硅酮纳米粒子（GdNPs）标记，而内皮脐静脉细胞（HUVECs）用柠檬酸稳定的磁赤铁矿纳米粒子（IONPs）标记，这两种粒子主要缩短支架和组织中水分子的 T-弛豫时间。通过在组织支架和体内进行 T-和 T2 加权 MRI，实现了双重细胞检测。