MRI 追踪负载超顺磁性氧化铁纳米颗粒的树突状细胞。

MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles.

机构信息

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China.

Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.

出版信息

Methods Mol Biol. 2020;2126:107-116. doi: 10.1007/978-1-0716-0364-2_10.

DOI:10.1007/978-1-0716-0364-2_10

PMID:32112383

Abstract

Cell tracking via MRI has drawn much attention recently for its sensitive, deep, and real-time properties and high spatial resolution. In a previous chapter, the labeling and tracking of superparamagnetic iron oxide (SPIO)-nanoparticle-loaded stem cells have been well summarized (Sykova et al., Methods Mol Biol 750:79-90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells.

摘要

细胞示踪的磁共振成像是一种新型的细胞示踪方法，具有灵敏度高、能进行深层和实时检测、空间分辨率高等特点，目前已受到广泛关注。在前面的章节中，已经对超顺磁性氧化铁（SPIO）纳米颗粒标记的干细胞的标记和示踪进行了很好的总结（Sykova 等人，Methods Mol Biol 750:79-90, 2011）。因此，在本章中，我们将主要关注 SPIO 纳米颗粒标记的树突状细胞的 MRI 示踪，并提供一种使用临床 3.0T MRI 扫描仪进行细胞标记和体内示踪的详细方案。值得注意的是，该方案也适用于其他类型的细胞。