Habashi Waseem, Bader-Farraj Amal, Shpack Nir, Beitlitum Ilan, May Hila, Sarig Rachel
Department of Oral Biology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel.
Department of Orthodontics, The Maurice and Gabriela Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel.
J Clin Med. 2021 Sep 30;10(19):4528. doi: 10.3390/jcm10194528.
Enamel erosion has become a common clinical finding that often impairs dental esthetics and function. In the current study, we aimed to implement the three-dimensional surface texture analysis (3DST) method to explore the protective effect of fluoride on surface texture prior to erosive conditions. For each of the 50 teeth used in this study, the polished buccal enamel surface was divided into three separate areas: the first area was untouched polished enamel, the two other surfaces were immersed in 0.3% citric acid for 30 s. One was treated with high-concentration (19,000 ppm) sodium fluoride (NaF) solution prior to acid attack, and the other had no treatment prior to acid exposure. Enamel surface texture and step height measurements were obtained using a high-resolution disk scanning confocal microscope, and SEM images were also acquired. Surfaces treated with fluoride showed fewer variations in 3-D surface texture parameters than the eroded surface compared to the control group ( = 0.001). This was in accordance with the SEM descriptive images. The findings indicate that pre-fluoridated enamel areas were less affected by the acid and showed similar features to the untouched enamel. Moreover, a protective effect of the fluoride treatment against irreversible enamel damage was noted as the surface loss (step-height) was significantly reduced ( = 0.03). The study showed that 3DST analysis is a valuable methodology for detecting and quantifying subtle differences between the surfaces. When exploring the combination of all surface texture parameters, it was revealed that the pre-fluoridated eroded enamel surfaces showed considerable similarity to the untouched enamel.
牙釉质侵蚀已成为一种常见的临床现象,常常损害牙齿美观和功能。在本研究中,我们旨在运用三维表面纹理分析(3DST)方法,探究氟化物在侵蚀条件发生前对表面纹理的保护作用。对于本研究中使用的50颗牙齿中的每一颗,其抛光的颊侧牙釉质表面被分为三个独立区域:第一个区域是未处理的抛光牙釉质,另外两个表面浸入0.3%的柠檬酸中30秒。其中一个在酸蚀前用高浓度(19,000 ppm)的氟化钠(NaF)溶液处理,另一个在酸暴露前未进行处理。使用高分辨率圆盘扫描共聚焦显微镜获取牙釉质表面纹理和台阶高度测量数据,并采集扫描电子显微镜(SEM)图像。与对照组相比,用氟化物处理的表面在三维表面纹理参数上的变化比侵蚀表面更少(P = 0.001)。这与SEM描述性图像一致。研究结果表明,预先氟化的牙釉质区域受酸的影响较小,并且与未处理的牙釉质具有相似特征。此外,由于表面损失(台阶高度)显著降低(P = 0.03),表明氟化物处理对不可逆的牙釉质损伤具有保护作用。该研究表明,3DST分析是检测和量化表面之间细微差异的一种有价值的方法。当探究所有表面纹理参数的组合时,发现预先氟化的侵蚀牙釉质表面与未处理的牙釉质表现出相当大的相似性。