Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China.
Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China.
Talanta. 2020 May 15;212:120744. doi: 10.1016/j.talanta.2020.120744. Epub 2020 Jan 13.
Accurate detection of viruses is of great significance in preventing further spreading of infections and developing appropriate clinical treatment. Herein, a fluorescence molecularly imprinted sensor based on a metal-organic framework with high selectivity and high sensitivity at concentrations down to the picomolar (pmol) level was developed to recognize Japanese encephalitis virus (JEV). In this work, zinc acrylate was used as the functional monomer to form molecularly imprinted polymers on the surface of a silicon-modified metal organic frameworks via free radical polymerization. Polyethylene glycol (PEG) was then used as a blocking agent to enhance the ability of polymers to specifically recognize the template virus. Under optimal experimental conditions, the polymers exhibit a wide range of detection, 50 pmol L to 1400 pmol L, within 20 min, a low detection limit (13 pmol L), and good selectivity (IF = 4.3). These advantages enable this molecularly imprinted (MIP) sensor for important practical application value and significance in the detection and prevention of viruses.
准确检测病毒对于防止感染进一步传播和制定适当的临床治疗方法具有重要意义。在此，开发了一种基于金属有机框架的荧光分子印迹传感器，该传感器具有高选择性和高灵敏度，可在低至皮摩尔（pmol）浓度下识别日本脑炎病毒（JEV）。在这项工作中，丙烯腈锌用作功能单体，通过自由基聚合在硅修饰的金属有机框架表面形成分子印迹聚合物。然后，聚乙二醇（PEG）用作封端剂，以增强聚合物特异性识别模板病毒的能力。在最佳实验条件下，聚合物在 20 分钟内表现出较宽的检测范围，为 50 pmol L 至 1400 pmol L，检测限低（13 pmol L），选择性好（IF=4.3）。这些优势使这种分子印迹（MIP）传感器在病毒的检测和预防方面具有重要的实际应用价值和意义。
