Guo Ting, Deng Qiliang, Fang Guozhen, Ma Liang, Wang Shuo
College of Food Science, Southwest University, Chongqing 400715, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 15;279:121460. doi: 10.1016/j.saa.2022.121460. Epub 2022 Jun 6.
A novel strategy for sensing protein was proposed through combining the high selectivity of molecular imprinting technology with the excellent upconversion fluorescence of upconversion nanoparticles (UCNPs) and high specific surface area of metal-organic frameworks (MOFs). Herein, the UCNPs acted as signal reporter and MOFs were introduced to increase the rate of mass transfer. The UCNPs@MIL-100 as support material was prepared via a step-by-step method. The imprinted material-coated UCNPs@MIL-100 (UCNPs@MIL-100@MIPs) were obtained by sol-gel technique. The results showed that as the increase of the template protein concentration, the fluorescence intensity of UCNPs@MIL-100@MIPs quenched gradually, and the imprinting factor was 2.90. The linear in the range of 1.00 to 8.00 μM, and the detection limit was 0.59 μM. Therefore, the novel optosensing material is very promising for future applications.
通过将分子印迹技术的高选择性与上转换纳米粒子（UCNPs）优异的上转换荧光以及金属有机框架（MOFs）的高比表面积相结合，提出了一种检测蛋白质的新策略。在此，UCNPs作为信号报告器，引入MOFs以提高传质速率。通过逐步法制备了UCNPs@MIL-100作为支撑材料。通过溶胶-凝胶技术获得了印迹材料包覆的UCNPs@MIL-100（UCNPs@MIL-100@MIPs）。结果表明，随着模板蛋白浓度的增加，UCNPs@MIL-100@MIPs的荧光强度逐渐猝灭，印迹因子为2.90。线性范围为1.00至8.00 μM，检测限为0.59 μM。因此，这种新型的光传感材料在未来应用中非常有前景。
