Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, D-30559, Hannover, Germany.
Institute of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Foundation, Bünteweg 2, D-30559 Hannover, Germany.
Res Vet Sci. 2020 Jun;130:26-32. doi: 10.1016/j.rvsc.2020.01.019. Epub 2020 Jan 23.
Aims of the present study were to define reference intervals for viscoelastic analyses of canine haemostasis using the ROTEM® delta analyser, and as a secondary aspect to determine the precision (repeatability) of this method. Blood samples from 125 clinically healthy dogs were included. Measurements were performed with commercially available activating reagents (ex-tem, in-tem and kaolin solution) as well as without activation. Additional fourfold measurements were done in 3 of the normal blood samples and in 3 samples with haemostatic alterations to evaluate the precision of the method. Coefficients of variation (CVs) for most of the ROTEM variables were < 10%. Clot formation time and maximum clot elasticity showed a wide inter-individual variation in comparison with alpha angle and maximum clot firmness. A multivariate analysis on various ROTEM parameters revealed particularly a significant influence of neuter status and a significant interrelationship between the factors sex and neuter status for measurements with different activating reagents. These results reflected the fact that significant differences occurred only between intact and neutered females, but not in males. No or only occasionally significant differences were found between groups of sex, age, and size. In conclusion, CVs demonstrated that the method delivers repeatable results in canine citrated whole blood. Established reference intervals should deliver valuable orientation for the evaluation of viscoelastic properties of clotting whole blood in dogs using the ROTEM delta analyser. Neuter status in females appeared to be the most relevant influencing factor and should be considered for the interpretation of ROTEM delta test results.
本研究的目的是定义使用 ROTEM® delta 分析仪进行犬止血的黏弹性分析的参考区间，并作为次要方面确定该方法的精密度（重复性）。纳入了 125 只临床健康的狗的血液样本。使用市售的激活试剂（ex-tem、in-tem 和高岭土溶液）以及不激活进行测量。在 3 个正常血液样本和 3 个止血异常的样本中进行了额外的 4 倍测量，以评估该方法的精密度。大多数 ROTEM 变量的变异系数（CVs）<10%。与 alpha 角和最大血凝块硬度相比，凝块形成时间和最大血凝块弹性显示出广泛的个体间变异性。对各种 ROTEM 参数的多元分析表明，特别是中性状态对测量结果有显著影响，并且性别和中性状态之间存在显著的相互关系，适用于不同的激活试剂。这些结果反映了一个事实，即仅在完整和中性化的雌性之间存在显著差异，但在雄性中不存在。在性别、年龄和体型组之间，未发现或仅偶尔发现差异。总之，CVs 表明该方法在犬枸橼酸盐全血中可提供重复性结果。建立的参考区间应为使用 ROTEM delta 分析仪评估犬凝血全血的黏弹性特性提供有价值的指导。中性状态在雌性中似乎是最相关的影响因素，在解释 ROTEM delta 测试结果时应考虑这一因素。
