Neonatal Clinical Care Unit, King Edward Memorial and Perth Children's Hospitals, Perth, Washington, Australia,
The UWA Centre for Child Health Research, Telethon Kids Institute, Perth, Washington, Australia,
Neonatology. 2020;117(3):365-368. doi: 10.1159/000506132. Epub 2020 Feb 28.
Salivary measurement of hormones and vitamins is gaining prominence as a minimally invasive procedure with the negligible potential for harm. We aimed to assess the utility of saliva for assessing vitamin A status in extremely preterm infants.
Paired saliva and blood samples were collected at 4 weeks of age from infants born <28 weeks of gestation using a proprietary polymer swab. Plasma retinol was measured using high-performance liquid chromatography, and salivary retinol was measured using enzyme-linked immunosorbent assay.
Thirty infants were recruited with a median (IQR) gestation and birth weight of 26.2 weeks (24.8-27.2) and 865 g (718-1,002), respectively. An adequate volume of saliva (>50 µL) was obtained in 68%. There was no significant correlation (Spearman's correlation coefficient = 0.16, p = 0.3) between individual plasma and salivary retinol levels. Bland-Altman analysis showed wide limits of agreement (-113 to +119%) between individual plasma and salivary retinol levels.
Individual vitamin A status cannot be determined reliably from saliva in extremely preterm infants using current collection materials and analysis techniques.
唾液测量激素和维生素作为一种微创程序，其潜在危害可以忽略不计，因此越来越受到关注。我们旨在评估唾液在评估极早产儿维生素 A 状况中的作用。
使用专利聚合物拭子在出生<28 周的婴儿出生后 4 周时采集唾液和血液样本。使用高效液相色谱法测量血浆视黄醇，使用酶联免疫吸附法测量唾液视黄醇。
共招募了 30 名婴儿，中位（IQR）胎龄和出生体重分别为 26.2 周（24.8-27.2）和 865 g（718-1,002）。有 68%的婴儿获得了足够量的唾液（>50 µL）。个体血浆和唾液视黄醇水平之间没有显著相关性（Spearman 相关系数=0.16，p=0.3）。Bland-Altman 分析显示个体血浆和唾液视黄醇水平之间的一致性界限很宽（-113 至+119%）。
使用当前的采集材料和分析技术，无法从极早产儿的唾液中可靠地确定个体维生素 A 状况。
