唾液评估极早产儿维生素 A 状态：一项诊断研究。

Saliva for Assessing Vitamin A Status in Extremely Preterm Infants: A Diagnostic Study.

机构信息

Neonatal Clinical Care Unit, King Edward Memorial and Perth Children's Hospitals, Perth, Washington, Australia,

The UWA Centre for Child Health Research, Telethon Kids Institute, Perth, Washington, Australia,

出版信息

Neonatology. 2020;117(3):365-368. doi: 10.1159/000506132. Epub 2020 Feb 28.

DOI:10.1159/000506132

PMID:32114572

Abstract

BACKGROUND

Salivary measurement of hormones and vitamins is gaining prominence as a minimally invasive procedure with the negligible potential for harm. We aimed to assess the utility of saliva for assessing vitamin A status in extremely preterm infants.

METHODS

Paired saliva and blood samples were collected at 4 weeks of age from infants born <28 weeks of gestation using a proprietary polymer swab. Plasma retinol was measured using high-performance liquid chromatography, and salivary retinol was measured using enzyme-linked immunosorbent assay.

RESULTS

Thirty infants were recruited with a median (IQR) gestation and birth weight of 26.2 weeks (24.8-27.2) and 865 g (718-1,002), respectively. An adequate volume of saliva (>50 µL) was obtained in 68%. There was no significant correlation (Spearman's correlation coefficient = 0.16, p = 0.3) between individual plasma and salivary retinol levels. Bland-Altman analysis showed wide limits of agreement (-113 to +119%) between individual plasma and salivary retinol levels.

CONCLUSION

Individual vitamin A status cannot be determined reliably from saliva in extremely preterm infants using current collection materials and analysis techniques.

摘要

背景

唾液测量激素和维生素作为一种微创程序，其潜在危害可以忽略不计，因此越来越受到关注。我们旨在评估唾液在评估极早产儿维生素 A 状况中的作用。

方法

使用专利聚合物拭子在出生<28 周的婴儿出生后 4 周时采集唾液和血液样本。使用高效液相色谱法测量血浆视黄醇，使用酶联免疫吸附法测量唾液视黄醇。

结果

共招募了 30 名婴儿，中位（IQR）胎龄和出生体重分别为 26.2 周（24.8-27.2）和 865 g（718-1,002）。有 68%的婴儿获得了足够量的唾液（>50 µL）。个体血浆和唾液视黄醇水平之间没有显著相关性（Spearman 相关系数=0.16，p=0.3）。Bland-Altman 分析显示个体血浆和唾液视黄醇水平之间的一致性界限很宽（-113 至+119%）。