Department of Life Science, Chung-Ang University, Seoul, Korea.
Department of Biological Science, Andong National University, Andong, Korea.
Plant Signal Behav. 2020 Apr 2;15(4):1734333. doi: 10.1080/15592324.2020.1734333. Epub 2020 Feb 29.
expression and RT-PCR analysis revealed that is predominantly expressed in shoots of Arabidopsis seedlings under light conditions. -overexpressed mutant produced more ethylene relative to the wild-type, which resulted in reduced growth of Arabidopsis seedlings. The abnormal growth of seedlings recurred after the application of Co ions, suggesting that is a functional necessary to regulate the growth and development of Arabidopsis seedlings. Exogenously-applied brassinosteroids (BRs) inhibited the expression of , and an enhanced expression was found in , a BR-deficient mutant. Additionally, expression of was decreased in (a -dominant mutant), implying that BR signaling negatively regulates expression via in Arabidopsis. In the intergenic region of , four E-boxes and a BR regulatory element (BRRE) are found. Electrophoretic mobility shift and chromatin immunoprecipitation assays showed that BZR1 binds directly to the BRRE in the putative promoter region of . By binding of BZR1 to BRRE, less ethylene was produced, which seems to regulate the growth and development of Arabidopsis seedlings.
表达和 RT-PCR 分析表明，在光照条件下， 主要在拟南芥幼苗的芽中表达。过表达突变体 产生的乙烯相对野生型更多，导致拟南芥幼苗生长受阻。在施用 Co 离子后，幼苗的异常生长再次出现，表明 是一个功能上必需的，以调节拟南芥幼苗的生长和发育。外源施加的油菜素内酯（BRs）抑制了 的表达，在 BR 缺陷突变体 中发现了增强的 表达。此外，在 （一个 -显性突变体）中， 的表达减少，这表明 BR 信号通过 在拟南芥中负调控 的表达。在 的基因间区，发现了四个 E-box 和一个 BR 调节元件（BRRE）。电泳迁移率变动和染色质免疫沉淀试验表明，BZR1 直接结合到 的推定启动子区域中的 BRRE。通过 BZR1 与 BRRE 的结合，产生的乙烯减少，这似乎调节拟南芥幼苗的生长和发育。
