Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
PeerJ. 2022 May 13;10:e13189. doi: 10.7717/peerj.13189. eCollection 2022.
Brassinolide (BR) plays an important role in plant growth, development, and the adaptation adversity process. Moreover, BRI1-EMS-SUPPRESSOR 1 ( genes are crucial transcription factors (TFs) in the BR signaling pathway. To realize the function of HbBES1 family is helpful to improve genetic resources for rubber tree breeding. Based on the rubber tree database, we used bioinformatics to characterize physicochemical properties, gene structure, -elements, and expression patterns. These results indicated that there were nine members in rubber tree, which we named to and divided into two groups (I and II) based on their genetic relationships. genes in the same group shared similar gene structures and motifs. -acting element analysis showed that the promoter sequences of genes contained many regulator elements that were related to hormone and stress, indicating that genes might be involved in the regulation of hormone and stress signal pathways. Our analysis of tissue specificity revealed that all of the nine members expressed highly in branches. Gene expression profiles under different hormone treatments showed that the gene family was induced to varying degrees under different hormones, and were extremely induced by ethylene (ETH). These results lay the foundation for further exploration of the molecular mechanism of the gene family, especially and , regulating plant stress tolerance in rubber tree.
油菜素内酯（BR）在植物的生长、发育和逆境适应过程中起着重要作用。此外，BRI1-EMS-SUPPRESSOR 1（BES1）基因是 BR 信号通路中的关键转录因子（TFs）。实现 HbBES1 家族的功能有助于提高橡胶树的遗传资源用于育种。基于橡胶树数据库，我们使用生物信息学方法对其理化性质、基因结构、顺式作用元件和表达模式进行了分析。结果表明，橡胶树中有 9 个成员，我们将其命名为 到 ，并根据遗传关系将其分为两组（I 和 II）。同一组中的 基因具有相似的基因结构和基序。顺式作用元件分析表明，基因启动子序列包含许多与激素和胁迫相关的调控元件，表明 基因可能参与激素和胁迫信号途径的调控。我们对组织特异性的分析表明，这 9 个 成员在树枝中都有高表达。不同激素处理下的基因表达谱显示，该基因家族在不同激素处理下被不同程度地诱导，和 被乙烯（ETH）极诱导。这些结果为进一步探讨 HbBES1 基因家族，特别是 和 ，在橡胶树中调节植物抗逆性的分子机制奠定了基础。
